Experimental Study on Anisotropic Strength and Deformation Behavior of a Coal Measure Shale under Room Dried and Water Saturated Conditions
Experimental Study on Anisotropic Strength and Deformation Behavior of a Coal Measure Shale under Room Dried and Water Saturated Conditions
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硫化氢通过一氧化氮介导维持大麦幼苗根部离子稳态来增强耐盐性
DOI：
10.1038/srep12516
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发表时间：
2015-01-01
影响因子：
1.6
通讯作者：
Zhang, Peng
中科院分区：
文献类型：
--
作者：
Cheng, Jingyi;Wan, Zhijun;Zhang, Peng
硫化氢（H2S）和一氧化氮（NO）是参与调节植物生理过程的信使分子。本文研究了H2S和NO参与大麦耐盐途径的信号网络。低浓度（50或100 μ M）的H2S供体NaHS对150 mM NaCl诱导的植物生长抑制具有显著的拯救作用，并通过减少K+净流出和增加内向整流钾通道（HvAKT1）和高亲和力K+吸收系统（HvHAK4）的基因表达来调节K +/Na+平衡。H_2S和NO通过增加质膜H~+-ATP酶（HvHA1）和Na~+/H~+逆向转运蛋白（HvSOS1）的转录水平，维持细胞质内较低的Na~+含量。H_2S和NO通过上调液泡膜Na~+/H~+逆向转运蛋白（HvVNHX 2）和H~+-ATP酶β亚基（HvVHA-β）的转录水平，以及增加液泡膜Na~+/H~+逆向转运蛋白（NHE 1）的蛋白表达，调节Na~+向液泡膜的分隔。H2S模拟硝普钠（SNP）的作用，通过增加NO的产生，而功能淬灭与NO清除剂的加入。这些结果表明，H2S通过维持离子稳态来提高耐盐性，这是由NO信号介导的。
Hydrogen sulfide (H2S) and nitric oxide (NO) are emerging as messenger molecules involved in the modulation of plant physiological processes. Here, we investigated a signalling network involving H2S and NO in salt tolerance pathway of barley. NaHS, a donor of H2S, at a low concentration of either 50 or 100 μM, had significant rescue effects on the 150 mM NaCl-induced inhibition of plant growth and modulated the K+/Na+balance by decreasing the net K+efflux and increasing the gene expression of an inward-rectifying potassium channel (HvAKT1) and a high-affinity K+uptake system (HvHAK4). H2S and NO maintained the lower Na+content in the cytoplast by increasing the amount of PM H+-ATPase, the transcriptional levels of PM H+-ATPase (HvHA1) and Na+/H+antiporter (HvSOS1). H2S and NO modulated Na+compartmentation into the vacuoles with up-regulation of the transcriptional levels of vacuolar Na+/H+antiporter (HvVNHX2) and H+-ATPase subunit β (HvVHA-β) and increased in the protein expression of vacuolar Na+/H+antiporter (NHE1). H2S mimicked the effect of sodium nitroprusside (SNP) by increasing NO production, whereas the function was quenched with the addition of NO scavenger. These results indicated that H2S increased salt tolerance by maintaining ion homeostasis, which were mediated by the NO signal.