Rhinovirus-induced interferon production is not deficient in well controlled asthma
Rhinovirus-induced interferon production is not deficient in well controlled asthma
复制标题
DOI：
10.1136/thoraxjnl-2012-202909
复制
发表时间：
2014-03-01
期刊：
影响因子：
10
通讯作者：
Johnston, Sebastian L.
中科院分区：
文献类型：
--
作者：
Sykes, Annemarie;Macintyre, Jonathan;Johnston, Sebastian L.
在哮喘患者的原代人支气管上皮细胞（HBECs）中，已经报道了鼻病毒（RV）缺陷诱导的干扰素(IFN)-β和IFN-λ的产生和RV复制的增加。这种缺陷在哮喘中有多普遍尚不清楚。此外，在原发性HBECs中，RV感染诱导的IFN亚型尚未得到全面研究。目的研究RV对异位性哮喘和健康人HBECs中IFN-α、IFN-β和IFN-λ的诱导作用及RV的复制。方法分别从感染RV16和RV1B的哮喘患者和健康对照组中获取shbecs，分别于8、24和48h采集细胞和上清液。ELISA法检测IFN蛋白表达，定量PCR法检测IFN mRNA和病毒RNA表达。在细胞上清液中评估病毒释放。结果RV在HBECs中诱导的IFN蛋白只有fn -β和IFN-λ，且IFN-λ蛋白的诱导量明显大于IFN-β。RV16在48小时诱导哮喘HBECs中IFN-λ1 mRNA显著增加；除此之外，哮喘患者与对照组在RV复制、I型或III型IFN蛋白或mRNA诱导方面无显著差异。结论RV感染HBECs诱导的IFN亚型主要为IFN-λ，其次为IFN-β。在本研究报告的控制良好的哮喘受试者的HBECs中，没有观察到RV诱导IFN缺陷或RV复制增加。
BackgroundDefective rhinovirus (RV)-induced interferon (IFN)-β and IFN-λ production and increased RV replication have been reported in primary human bronchial epithelial cells (HBECs) from subjects with asthma. How universal this defect is in asthma is unknown. Additionally, the IFN subtypes induced by RV infection in primary HBECs have not been comprehensively investigated.ObjectiveTo study RV induction of IFN-α, IFN-β and IFN-λ and RV replication in HBECs from subjects with atopic asthma and healthy controls.MethodsHBECs were obtained from subjects with asthma and healthy controls and infected with RV16 and RV1B, and cells and supernatants harvested at 8, 24 and 48h. IFN proteins were analysed by ELISA and IFN mRNA and viral RNA expression by quantitative PCR. Virus release was assessed in cell supernatants.ResultsIFN-β and IFN-λ were the only IFNs induced by RV in HBECs and IFN-λ protein induction was substantially greater than IFN-β. Induction of IFN-λ1 mRNA by RV16 at 48h was significantly greater in HBECs from subjects with asthma; otherwise there were no significant differences between subjects with asthma and controls in RV replication, or in induction of type I or III IFN protein or mRNA.ConclusionsIFN-λ and, to a lesser degree, IFN-β are the major IFN subtypes induced by RV infection of HBECs. Neither defective IFN induction by RV nor increased RV replication was observed in the HBECs from subjects with well controlled asthma reported in this study.