MicroRNA expression profiles of serum from patients before and after chemotherapy.
MicroRNA expression profiles of serum from patients before and after chemotherapy.
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化学疗法前后患者血清的microRNA表达谱。
DOI：
10.1016/j.gdata.2015.08.018
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发表时间：
2015-12
期刊：
影响因子：
--
通讯作者：
Bissels U
中科院分区：
文献类型：
--
作者：
Diener Y;Walenda T;Jost E;Brümmendorf TH;Bosio A;Wagner W;Bissels U
化疗后血液和免疫系统的恢复需要造血干细胞和祖细胞（HPSCs）的增殖。研究表明，化疗后血清中系统性释放因子可刺激体外HSPC扩增。我们想知道血清中循环的microRNAs （miRNAs）是否可以解释这种效应。因此，我们比较了恶性血液病患者化疗前后血清miRNA的表达谱。除了化疗后miRNA表达普遍下降外，我们还发现化疗前后血清中有23种miRNA表达显著差异。miRNA微阵列数据可在NCBI的Gene Expression Omnibus (GEO) Series登录号GSE57570上获得。在这里，我们提供了miRNA微阵列和数据分析的详细协议。
Recovery of the blood and immune system after chemotherapy requires proliferation of hematopoietic stem and progenitor cells (HPSCs). It has been shown that systemically released factors in serum after chemotherapy stimulate HSPC expansion in vitro. We wondered if microRNAs (miRNAs) circulating in serum could account for this effect. Therefore, we compared the miRNA expression profiles of serum from patients with hematologic malignancies before and after chemotherapy. In addition to a general decrease in miRNA expression after chemotherapy, we found 23 miRNAs to be significantly differentially expressed in serum before versus after chemotherapy. The miRNA microarray data are available at NCBI's Gene Expression Omnibus (GEO) Series accession number GSE57570. Here, we provide a detailed protocol of the miRNA microarray and data analysis.