Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. trees
Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. trees
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DOI：
10.1016/j.foodchem.2007.01.019
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发表时间：
2007-01-01
期刊：
影响因子：
8.8
通讯作者：
Przybylski, Roman
中科院分区：
文献类型：
--
作者：
Sultana, Bushra;Anwar, Farooq;Przybylski, Roman
对四种不同树木（印楝、矮树、阿拉伯金合欢、和Eugenia jambolana Lam.）以80%甲醇、80%乙醇和80%丙酮（溶剂：水，80：20 v/v）为溶剂，测定了其抗氧化活性、总酚（TP）和总黄酮（TF）含量。通过测定还原力、亚油酸体系过氧化反应抑制率和2，2 ′-二苯基-1-苦基肼自由基（DPPH）清除率来测定其抗氧化活性。不同树皮提取物的TP、TF、抑制亚油酸氧化和清除DPPH自由基的活性差异显著（P < 0.05）。然而，观察到还原能力的微小变化。所有树皮提取物的总酚含量范围很广，没食子酸当量为7.8-16.5，总黄酮含量范围为1.59-4.93。10 mg/mL浸提液浓度下的还原能力范围为1.34 - 1.87。不同树皮提取物对亚油酸氧化的抑制率为44-90%，对DPPH自由基的清除率为49%-87%。抗氧化成分的提取效果依次为乙醇>甲醇>丙酮。各提取物对TP和DPPH自由基的清除活性具有良好的相关性。A.尼罗树皮的TP含量最高，为9.2 ~ 16.5g/100 g，而通过抑制亚油酸氧化测定的AA含量最高的是E.詹博拉纳湖同一树种清除DPPH自由基能力和还原力最高。不同抗氧化剂测定结果之间的相关性虽然揭示了一些测定之间的强相关性，但是，可能需要一些不同的方法来充分评估特定植物材料的体外抗氧化活性。(c)2007爱思唯尔有限公司版权所有。
Barks extracts of four different trees (Azadirachia indica, Terminalia aijuna, Acacia nilotica., and Eugenia jambolana Lam.) in three different solvents 80% methanol, 80% ethanol, and 80% acetone (solvent:water, 80:20 v/v) were evaluated for their antioxidant activity, total phenolic (TP), and total flavonoids (TF) contents. Antioxidant activity (AA) was determined by measuring reducing power, inhibition of peroxidation using linoleic acid system and 2,2'-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging activity. Significant (P < 0.05) differences were observed in the TP, TF, inhibition of linoleic acid oxidation and DPPH scavenging activity of different bark extracts. Nevertheless, minute variation was observed in reducing power. All the bark extracts exhibited wide range of total phenolic, 7.8-16.5 gallic acid equivalents and total flavonoid contents, 1.59-4.93 catechin equivalents. Reducing power at 10 mg/mL extract concentration ranged from 1.34 to 1.87. Different bark extracts inhibited oxidation of linoleic acid by 44-90% while DPPH radical scavenging activity ranged from 49% to 87%. Extraction efficacy of components with antioxidative properties was lowering in the following order: ethanol > methanol > acetone. Good correlation was observed between TP and DPPH scavenging activity among the extracts. A. nilotica bark had the highest amounts of TP, ranging from 9.2 to 16.5 g/100 g, while the highest AA as measurement by inhibition of linoleic acid oxidation is offered by bark from E. jambolana Lam. The same tree showed the highest DPPH scavenging activity and reducing power. The correlation among the results of different antioxidant assays although revealed a strong relationship between some of the assays, however, a number of different methods may be necessary to adequately assess the in vitro antioxidant activity of a specific plant material. (c) 2007 Elsevier Ltd. All rights reserved.