Resveratrol attenuates the inflammatory reaction induced by ischemia/reperfusion in the rat heart
Resveratrol attenuates the inflammatory reaction induced by ischemia/reperfusion in the rat heart
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DOI：
10.3892/mmr.2014.2090
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发表时间：
2014-06-01
影响因子：
3.4
通讯作者：
Liu, Ya
中科院分区：
文献类型：
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作者：
Cong, Xiaoqiang;Li, Ying;Liu, Ya
白藜芦醇（resveratrol, Res）在缺血/再灌注引起的炎症中的作用尚不清楚。本研究的目的是研究Res是否在缺血/再灌注损伤大鼠心脏模型中调节中性粒细胞积累和肿瘤坏死因子- α (tnf - α)诱导。随机给予假手术、心肌缺血/再灌注（MI/R）、MI/R + Res、MI/R + Res + l - ng -硝基精氨酸甲酯（L-NAME）和MI/R + Res +亚甲基蓝（MB）。结果表明，与MI/R相比，Res降低了心肌梗死面积、心肌髓过氧化物酶水平、血清肌酐激酶和乳酸脱氢酶水平，降低了血清和心肌tnf - α的产生。Res的所有作用均被L-NAME（一种一氧化氮合成酶抑制剂）和MB（一种环磷酸鸟苷（cGMP）抑制剂）所抑制。因此，Res具有心脏保护和抗炎作用。这些作用可能与心肌心肌中NO生成的增加、中性粒细胞积累的抑制、tnf - α诱导和cGMP信号通路有关。
The role of resveratrol (Res) in inflammation induced by ischemia/reperfusion is not well understood. The aim of the present study was to investigate whether Res modulates neutrophil accumulation and tumor necrosis factor-alpha (TNF-alpha) induction in an ischemia/reperfusion-injured rat heart model. The rats were randomly exposed to sham surgery, myocardial ischemia/reperfusion (MI/R) alone, MI/R + Res, MI/R + Res + L-NG-nitroarginine methyl ester (L-NAME) and MI/R + Res + methylene blue (MB). The results demonstrated that compared with MI/R, Res reduced the myocardial infarct area, myocardial myeloperoxidase levels, serum creatinine kinase and lactate dehydrogenase levels, and serum and myocardial TNF-alpha production. All the effects of Res demonstrated were inhibited by L-NAME (a nitric oxide (NO) synthase inhibitor) and MB [a cyclic guanosine monophosphate (cGMP) inhibitor]. Thus, Res produces cardioprotective and anti-inflammatory effects. These effects may be associated with an increase in NO production, the inhibition of neutrophil accumulation, TNF-alpha induction and cGMP signaling pathways in myocardium subjected to MI/R.