Analysis of key genes and signaling pathways involved in Helicobacter pylori-associated gastric cancer based on The Cancer Genome Atlas database and RNA sequencing data
Analysis of key genes and signaling pathways involved in Helicobacter pylori-associated gastric cancer based on The Cancer Genome Atlas database and RNA sequencing data
复制标题
基于癌症基因组图谱数据库和RNA测序数据分析幽门螺杆菌相关胃癌关键基因和信号通路
DOI：
10.1111/hel.12530
复制
发表时间：
2018-10-01
期刊：
影响因子：
4.4
通讯作者：
Zhu, Yin
中科院分区：
文献类型：
--
作者：
Hu, Yi;He, Cong;Zhu, Yin
背景
幽门螺杆菌（H. pylori）感染与胃癌的发生相关，尽管其机制尚不清楚。在此，本研究旨在基于癌症基因组图谱（TCGA）数据库和RNA测序分析来阐明幽门螺杆菌致病过程中所涉及的关键基因和信号通路。
材料与方法
通过生物信息学分析来自TCGA数据库的49例胃癌样本（16例有幽门螺杆菌感染，33例无幽门螺杆菌感染）以及35例癌旁正常样本。在18例胃癌（GC）样本（9例有幽门螺杆菌感染，9例无幽门螺杆菌感染）中验证幽门螺杆菌阳性和幽门螺杆菌阴性患者之间的差异表达基因，这些样本采用RNA测序进行分析。进行生存分析以探索差异表达基因与预后之间的关联。进行生物信息学分析以确定与幽门螺杆菌相关的信号通路。
结果
来自TCGA数据库和RNA测序的临床特征基线水平在幽门螺杆菌阳性和幽门螺杆菌阴性胃癌组之间无差异（P>0.05）。在TCGA数据库和RNA测序数据中，TP53在幽门螺杆菌阳性组中均显示上调，其在胃癌组织中的表达也高于癌旁正常组织（P
BackgroundHelicobacter pylori (H. pylori) infection is associated with the development of gastric cancer, although the mechanism is unclear. Herein, this study aimed to clarify the key genes and signaling pathways involved in H. pylori pathogenesis based on The Cancer Genome Atlas (TCGA) database and RNA sequencing analysis.Materials and MethodsForty-nine gastric cancer samples (16 with H. pylori and 33 without H. pylori) and 35 cancer-adjacent normal samples from TCGA database were analyzed by bioinformatics. The differentially expressed genes between H. pylori-positive and H. pylori-negative patients were verified in 18 gastric cancer (GC) samples (9 with H. pylori and 9 without H. pylori), which were analyzed using RNA sequencing. Survival analysis was carried out to explore associations between the differentially expressed genes and prognosis. Bioinformatics analysis was performed to determine the signaling pathways associated with H. pylori.ResultsThe baseline level of clinical features from TCGA database and RNA sequencing showed no differences between the H. pylori-positive and H. pylori-negative GC groups (P>0.05). TP53 was shown to be upregulated in the H. pylori-positive group in both TCGA database and RNA sequencing data, which also showed higher expression in the GC tissues than in adjacent normal tissues (P