Leydig cell loss and spermatogenic arrest in platelet-derived growth factor (PDGF)-A-deficient mice.
Leydig cell loss and spermatogenic arrest in platelet-derived growth factor (PDGF)-A-deficient mice.
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DOI：
10.1083/jcb.149.5.1019
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发表时间：
2000-05-29
期刊：
影响因子：
--
通讯作者：
Betsholtz C
中科院分区：
文献类型：
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作者：
Gnessi L;Basciani S;Mariani S;Arizzi M;Spera G;Wang C;Bondjers C;Karlsson L;Betsholtz C
发现血小板衍生生长因子(PDGF)- a缺乏的雄性小鼠出现睾丸大小进行性缩小，间质细胞丢失和生精停止。在正常小鼠中，PDGF-A和pdgf - r - α分别在精质上皮和间质细胞中呈阳性表达。PDGF-A - / -小鼠的睾丸缺陷，结合PDGF-A和pdgf - r - α的正常发育表达，表明PDGF-A基因通过上皮-间质信号传导对间质细胞谱系的发育至关重要。这些发现表明PDGF-A可能在参与雄性性腺分化的一系列基因中发挥作用。突变小鼠的间质细胞丢失和生精损伤使人想起人类睾丸衰竭的病例。
Platelet-derived growth factor (PDGF)- A–deficient male mice were found to develop progressive reduction of testicular size, Leydig cells loss, and spermatogenic arrest. In normal mice, the PDGF-A and PDGF-Rα expression pattern showed positive cells in the seminiferous epithelium and in interstitial mesenchymal cells, respectively. The testicular defects seen in PDGF-A−/− mice, combined with the normal developmental expression of PDGF-A and PDGF-Rα, indicate that through an epithelial-mesenchymal signaling, the PDGF-A gene is essential for the development of the Leydig cell lineage. These findings suggest that PDGF-A may play a role in the cascade of genes involved in male gonad differentiation. The Leydig cell loss and the spermatogenic impairment in the mutant mice are reminiscent of cases of testicular failure in man.