Studies on the nature of the hypothalamic estradiol-concentrating mechanism in the male and female rat.
Studies on the nature of the hypothalamic estradiol-concentrating mechanism in the male and female rat.
复制标题
雄性和雌性大鼠下丘脑雌二醇浓缩机制性质的研究。
作者：
K. Korach;T. G. Muldoon
Hypothalamic cytosol receptor binding of gonadal steroids was studied in both male and female intact and castrate rats. Assessment of specific binding by the protamine precipitation technique indicated a capacity of 1.34–2.03 × 10−14 moles estradiol bound/mg protein among the animal models studied. Although testosterone and 5α-dihydrotestosterone (17β-hydroxy-5αandrostane-3-one) also showed a small amount of binding to cytosol, further experimentation showed that this binding did not obey the characteristic saturability phenomena of high affinity receptor systems as did the estradiol binding system. Quantification of the estradiol receptor interaction was estimated as 1.48 and 1.55 × 10−14 moles/mg protein for male and female, respectively, by gel filtration, with elution of the complex in a peak just following the void volume. A specific 8S-sedimenting estradiol binding component was demonstrated on sucrose density gradient centrifugation. Patterns from male and female cytosol samples were very similar a...
