The essential two-component regulatory system encoded by yycF and yycG modulates expression of the ftsAZ operon in Bacillus subtilis
The essential two-component regulatory system encoded by yycF and yycG modulates expression of the ftsAZ operon in Bacillus subtilis
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DOI：
10.1099/00221287-146-7-1573
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发表时间：
2000-07-01
期刊：
影响因子：
--
通讯作者：
Ogasawara, N
中科院分区：
文献类型：
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作者：
Fukuchi, K;Kasahara, Y;Ogasawara, N
现在已经在细菌中发现了基本的双组分系统。最近有报道称，枯草芽孢杆菌yycF基因编码一种反应调节因子，其在金黄色葡萄球菌中的同源物对细胞生长至关重要，尽管它们控制的基因尚未被确定。在构建基因组序列计划中新发现的基因敲除突变库的过程中，也注意到枯草芽孢杆菌中yycF调控基因及其同源激酶基因yycG的本质。我们发现yycG可以在YycF蛋白的活性形式存在的情况下被删除，从而提示yycG和YycF之间存在直接的相互作用。当YycF调节因子在枯草芽孢杆菌中过量产生时，会产生微型细胞并减少细胞长度。这些观察结果导致发现YycF的过量产生上调了细胞分裂操纵子ftsAZ的P1启动子的表达。此外，YycF蛋白在体外与Pt启动子区域结合。这些结果清楚地表明，yycF和yycG基因编码的必需双组分调控系统具有调节枯草芽孢杆菌ftsAZ操纵子表达的潜力。
Essential two-component systems are now being identified in bacteria. The Bacillus subtilis yycF gene encoding a response regulator, and its orthologue in Staphylococcus aureus, were reported recently to be essential for cell growth, although genes under their control have yet to be identified. The essential nature of the yycF regulator gene and its cognate kinase gene, yycG, in B. subtilis was also noted during the course of construction of a knockout mutant bank of newly identified genes in the genome sequence project. It was found that yycG could be deleted in the presence of an active form of the YycF protein, thereby suggesting direct interaction between YycG and YycF. Production of mini-cells and reduction in cell length occurred when the YycF regulator was overproduced in B. subtilis. These observations led to the finding that YycF overproduction up-regulated the expression from the P1 promoter of the cell division operon, ftsAZ. In addition, the YycF protein binds to the Pt promoter region in vitro. These results clearly indicate that the essential two-component regulatory system encoded by yycF and yycG genes has the potential to modulate expression of the ftsAZ operon in B. subtilis.