Oral administration of Cimicifuga racemosa extract affects immobilization stress-induced changes in murine cerebral monoamine metabolism
Oral administration of Cimicifuga racemosa extract affects immobilization stress-induced changes in murine cerebral monoamine metabolism
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DOI：
10.2220/biomedres.33.133
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发表时间：
2012-04-01
影响因子：
1.2
通讯作者：
Kitagawa, Yasushi
中科院分区：
文献类型：
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作者：
Nadaoka, Isao;Yasue, Masaaki;Kitagawa, Yasushi
我们研究了Cimicifuga racemosa（CR）植物提取物对急性束缚应激小鼠脑内单胺类物质去甲肾上腺素（NE）、多巴胺（DA）和5-羟色胺（5-HT）及其代谢产物3-甲氧基-4-羟基苯乙二醇（MHPG）、3，4-二羟基苯乙酸（DOPAC）和5-羟基吲哚乙酸（5-HIAA）和血浆皮质酮水平变化的影响。单次经口给予CR提取物（1，000 mg/kg）可显著降低因强制固定而升高的血浆皮质酮水平。急性制动应激引起下丘脑，海马和皮质中相应的胺代谢物比例的显着变化;然而，CR提取物治疗显着减弱下丘脑中的MHPG/NE变化，以及大脑各区域中的5-HIAA/5-HT变化。我们的研究结果表明，CR提取物相互作用，不仅与下丘脑-垂体-肾上腺（HPA）轴，但也与交感肾上腺髓质（SAM）系统在应激条件下。因此，CR提取物可以通过抑制脑中胺与代谢物比例的变化来减轻急性应激反应。
We investigated the effects of Cimicifuga racemosa (CR) plant extracts on the changes in levels of the cerebral monoamines norepinephrine (NE), dopamine (DA), and serotonin (5-HT), the respective metabolites 3-methoxy-4-hydroxyphenylglycol (MHPG), 3,4-dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIAA), and plasma corticosterone in mice subjected to acute immobilization stress. Single oral administration of the CR extract (1,000 mg/kg) significantly attenuated plasma corticosterone levels that had been increased as a result of enforced immobilization. Acute immobilization stress caused significant changes in the corresponding amine-to-metabolite ratios in the hypothalamus, hippocampus, and cortex; however, CR-extract treatment significantly attenuated the MHPG/NE change in the hypothalamus, and the 5-HIAA/5-HT changes in each region of the brain. Our results suggest that the CR extract interacts not only with the hypothalamic-pituitary-adrenal (HPA) axis but also with the sympathetic adrenomedullary (SAM) system under stress conditions. Thus the CR extract can alleviate acute stress responses by suppressing the changes of amine-to-metabolite ratio in brain.