Demonstration of the human herpesvirus 6-induced DNA polymerase and DNase.
Demonstration of the human herpesvirus 6-induced DNA polymerase and DNase.
复制标题
人类疱疹病毒 6 诱导的 DNA 聚合酶和 DNase 的演示。
DOI：
10.1016/0042-6822(89)90238-9
复制
发表时间：
1989
期刊：
影响因子：
3.7
通讯作者：
Glaser,R
中科院分区：
文献类型：
--
作者：
Williams,MV;Ablashi,DV;Salahuddin,SZ;Glaser,R
与模拟感染的细胞相比，用人类疱疹病毒 6 (HHV6) 感染 HSB-2 细胞，DNA 聚合酶活性水平增加约 51 倍，DNA 酶活性水平增加 4.44 倍。受感染细胞中胸苷激酶、尿嘧啶-DNA糖基化酶或脱氧尿苷三磷酸核苷酸水解酶活性没有增加。 HHV6 诱导的 DNase 和 DNA 聚合酶活性可根据免疫特异性与正常细胞对应物区分开来，而在 DNA 聚合酶的情况下，可根据电泳迁移的差异来区分。血清学研究还表明，抗血清不仅对 HHV6 具有反应性，而且对 Epstein-Barr 病毒也具有反应性。
Infection of HSB-2 cells with human herpesvirus 6 (HHV6) results in an approximately 51-fold increase in the level of DNA polymerase activity and a 4.44-fold increase in the level of DNase activity when compared to mock-infected cells. There was no increase in thymidine kinase, uracil-DNA glycosylase, or deoxyuridine triphosphate nucleotidohydrolase activities in the infected cells. The HHV6-induced DNase and DNA polymerase activities could be distinguished from their normal cellular counterparts on the basis of immunological specificities and in the case of DNA polymerase based upon differences in electrophoretic migration. Serological studies also demonstrated reactivity of the antisera not only for HHV6 but also for Epstein-Barr virus.