Morphokinetic analysis of cleavage stage embryos and its relationship to aneuploidy in a retrospective time-lapse imaging study
Morphokinetic analysis of cleavage stage embryos and its relationship to aneuploidy in a retrospective time-lapse imaging study
复制标题
DOI：
10.1007/s10815-014-0372-3
复制
发表时间：
2015-01-01
影响因子：
3.1
通讯作者：
Budak, Erdal
中科院分区：
文献类型：
--
作者：
Chawla, Monika;Fakih, Michael;Budak, Erdal
利用延时成像和CGH微阵列分析染色体正常和非整倍体胚胎的形态动力学参数差异，这项回顾性队列研究包括接受IVF治疗和植入前性别选择遗传学诊断的患者。在发育的第3天，选择在具有延时成像系统（EmbryoScope）的培养箱中培养的总共460个胚胎进行活检。随后，进行CGH微阵列分析用于24条染色体的非整倍体筛选。动力学参数包括第二极体出现时间（tPB 2）、原核出现时间（tPNa）、原核消失时间（tPBf）、分裂为2（t2）、3（t3）、4（t4）、5（t5）个细胞的时间、第二和第三细胞周期的长度（CC 2 = t3 t2，CC 3 =t5-t3），从2到4个细胞的细胞分裂同步（S2=t4-t3）和间隔t5-t2进行分析以比较染色体正常和异常胚胎。t5-t2在正常和异常胚胎之间存在显著差异。由于动力学行为的差异，延时成像形态动力学可能在早期预测非整倍体胚胎中发挥作用，这可能有助于改善临床结果。
To analyze differences in morphokinetic parameters of chromosomally normal and aneuploid embryos utilizing time-lapse imaging and CGH microarray analysis.This retrospective cohort study included patients undergoing IVF treatment and preimplantation genetic diagnosis for sex selection. A total of 460 embryos cultured in incubators with time-lapse imaging system (EmbryoScope) were selected for biopsy on day 3 of development. Subsequently, CGH microarray analysis was performed for aneuploidy screening of 24 chromosomes. Kinetic parameters including time for appearance of second polar body (tPB2), time of pronuclei appearance (tPNa), time of pronuclei fading (tPBf), time to division to 2(t2), 3(t3), 4(t4), 5(t5) cells, length of second and third cell cycle (CC2= t3 t2, CC3=t5-t3), synchrony of cell division from 2 to 4 cells (S2=t4-t3) and interval t5-t2 were analyzed to compare chromosomally normal and abnormal embryos.The mean time durations for tPNf, t2, t5, CC2, CC3, t5-t2 differed significantly between normal and abnormal embryos.Time-lapse imaging morphokinetics may play a role in early prediction of aneuploid embryos due to differences in kinetic behavior that may aid in improving clinical outcome.