Ultrastructural and histochemical study on the epithelial differentiation to Paneth cells in the rat ascending colon
Ultrastructural and histochemical study on the epithelial differentiation to Paneth cells in the rat ascending colon
复制标题
大鼠升结肠上皮向潘氏细胞分化的超微结构和组织化学研究
作者：
Mantani;Y.;Nishida;M.;Yuasa;H.;Yamamoto;K.;Takahara;E.;Omotehara;T.;Udayanga;K. G. S.;Kawano;J.;Yokoyama;T.;Hoshi;N. and Kitagawa;H.
潘氏细胞（PC）有助于宿主防御小肠中的固有细菌。我们在大鼠升结肠中发现了潘氏细胞样细胞（PLC），但PLC的性质从未阐明。因此，本研究旨在阐明PLC的细胞学特征，并讨论其细胞分化。PLC定位于肠隐窝的基部，尤其是近端结肠淋巴组织中的滤泡相关肠隐窝，但很少在升结肠的普通肠隐窝中发现。PLC具有特定的颗粒，具有高电子密度的核心和晕圈，以及小肠中的PC。PLCs分泌颗粒PAS反应、溶菌酶和可溶性磷脂酶A2阳性，Alcian蓝染色、β防御素-1和β防御素-2及PC阴性。此外，在PLC附近偶尔发现具有PLC特异性颗粒和类似于杯状细胞（GC）的粘液颗粒的中间细胞。中间细胞范围从富含粘液颗粒的杯状细胞样细胞到几乎没有粘液颗粒的PLC样细胞。细胞凝聚和碎裂仅见于PLC，而未见于中间细胞或GC。在大鼠升结肠近端结肠淋巴组织的肠隐窝中，PLC可能是由GC经中间细胞转化而成，并最终凋亡而死亡，被鉴定为PC。Anat Rec，297：1462-1471，2014。© 2014 Wiley Periodicals，Inc.
Paneth cells (PCs) contribute to the host defense against indigenous bacteria in the small intestine. We found Paneth cell‐like cells (PLCs) in the rat ascending colon, but the nature of PLCs is never clarified. Therefore, the present study aimed to clarify the cytological characteristics of PLCs and discuss their cellular differentiation. PLCs were localized in the bases of intestinal crypts, especially follicle‐associated intestinal crypts in proximal colonic lymphoid tissue, but were very seldom found in the ordinary intestinal crypts of the ascending colon. PLCs possessed specific granules with highly electron‐dense cores and haloes, as well as PCs in the small intestine. The secretory granules of PLCs were positive for PAS reaction, lysozyme and soluble phospholipase A2, but negative for Alcian blue staining, β‐defensin‐1 and ‐2, as well as the ones of PCs. Furthermore, intermediate cells possessing both the PLC‐specific granules and the mucus granules similar to those of goblet cells (GCs) were occasionally found in the vicinity of PLCs. Intermediate cells ranged from goblet cell‐like cells rich in mucus granules to PLC‐like cells with few mucus granules. The cellular condensation and fragmentation were exclusively found in PLCs but never seen in intermediate cells or GCs. The PLCs, which were identified as PC, were suggested to be transformed from GCs through intermediate cells and finally to die by apoptosis in intestinal crypts of proximal colonic lymphoid tissue in the rat ascending colon. Anat Rec, 297:1462–1471, 2014. © 2014 Wiley Periodicals, Inc.