DISTRIBUTION OF ACETYL CHOLINESTERASE IN HIPPOCAMPAL REGION OF GUINEA-PIG .1. ENTORHINAL AREA, PARASUBICULUM, AND PRESUBICULUM
DISTRIBUTION OF ACETYL CHOLINESTERASE IN HIPPOCAMPAL REGION OF GUINEA-PIG .1. ENTORHINAL AREA, PARASUBICULUM, AND PRESUBICULUM
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DOI：
10.1007/bf00325634
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发表时间：
1971-01-01
期刊：
影响因子：
--
通讯作者：
BLACKSTAD, TW
中科院分区：
文献类型：
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作者：
GENESERJENSEN, FA;BLACKSTAD, TW
1.乙酰胆碱酯酶的分布在豚鼠海马区的三个区域进行了检测，内嗅区、下托旁和前下托。2.甲醛固定的连续冰冻切片和未固定的冰冻切片进行改良的Koelle硫代胆碱法。异丙嗪用于抑制非特异性胆碱酯酶。根据Karnovsky和Roots（1964）的铁氰化铜方法处理一系列切片。3.在每个区域中，获得的染色显示出特定的分层模式，在过渡到邻近区域时停止。在豚鼠和大鼠中，AChE的分布与上述结果相比较。4.皮层深部的三个区的AChE分布相似，均为染色较深的Ⅳ层、较浅的Ⅴ层和较深的Ⅵ层。一个称为内侧部，有一个非常明显的沉淀分层;在另一个称为外侧部，有一个不同的，不太明显的分层。在内侧部相对缺乏酶在第三层和丰富的第四层是最显着的特点。与大鼠的相似性是相当大的，旁核由一条非常窄的皮质带组成，比大鼠的小。其I-III层构成了一个楔形的领域非常丰富的AChE。大多数水平的前下托比旁下托宽数倍，比大鼠宽得多。组织化学上，它不同于大鼠的前下托，特别是第三层的乙酰胆碱酯酶比其周围的要差得多。
1.The distribution of acetyl cholinesterase has been examined in the guinea pig in three areas belonging to the hippocampal region, viz., the entorhinal area, the parasubiculum, and the presubiculum.2.Formaldehyde-fixed serial frozen sections and unfixed cryostat sections were subjected to a modification of the Koelle thiocholine method. Ethopropazine was used to suppress non-specific cholinesterase. One series of sections was processed according to the copper ferricyanide method of Karnovsky and Roots (1964).3.In each area the obtained staining displayed a specific pattern of stratification, ceasing at the transition to neighbouring areas. The distribution in the guinea pig has been compared with that in the rat, previously described.4.The deep part of the cortex was similar with regard to AChE in the three areas, comprising a well-stained layer IV, a paler layer V, and a moderately dark layer VI.5.In the entorhinal area two subfields were discernible. One termed pars medialis has a very distinct stratification of precipitate; in the other, pars lateralis, a different and much less conspicuous stratification is seen. In pars medialis relative absence of enzyme in layer III and abundance in layer IV are the most striking features. The similarity with the rat is considerable.The parasubiculum consists of a very narrow strip of cortex, smaller than in the rat. Its layers I-III constitute a wedgeshaped field very rich in AChE. The presubiculum at most levels is several times wider than the parasubiculum and much wider than in the rat. Histochemically, it differs from the presubiculum of the rat, particularly by layer III being much poorer in AChE than its surroundings.