Immunochemical Detection of α-Synuclein Autoantibodies in Parkinson's Disease: Correlation between Plasma and Cerebrospinal Fluid Levels
Immunochemical Detection of α-Synuclein Autoantibodies in Parkinson's Disease: Correlation between Plasma and Cerebrospinal Fluid Levels
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DOI：
10.1021/acschemneuro.7b00063
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发表时间：
2017-06-01
影响因子：
5
通讯作者：
Morozova-Roche, Ludmilla A.
中科院分区：
文献类型：
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作者：
Horvath, Istvan;Iashchishyn, Igor A.;Morozova-Roche, Ludmilla A.
帕金森病（PD）淀粉样蛋白，α-突触核蛋白的自身抗体，被认为是一个前瞻性的生物标志物的早期疾病诊断，但在以前的报告中存在不一致的，可能与PD状态。因此，通过酶联免疫吸附试验（ELISA）检查了60名个体（包括最近诊断为轻度和中度PD的PD患者和年龄匹配的对照组）的血浆和脑脊液（CSF）。非参数统计用于数据分析。我们发现，与对照组相比，轻度PD患者血浆和CSF中α-突触核蛋白自身抗体水平显著升高，中度PD患者则有所降低。受试者操作特征和效应量分析证实了血浆和CSF中α-突触核蛋白抗体的诊断能力。我们首次显示了轻度、中度和合并PD组的血浆和CSF α-突触核蛋白抗体水平之间的相关性。这表明α-突触核蛋白抗体作为PD生物标志物的潜力以及其在血浆和CSF中同时分析的诊断能力增加
Autoantibodies to Parkinson's disease (PD) amyloidogenic protein, a-synuclein, were recognized as a prospective biomarker for early disease diagnostics, yet there is inconsistency in previous reports, potentially related to PD status. Therefore, plasma and cerebrospinal fluid (CSF) of the cross-sectional cohort of 60 individuals, including recently diagnosed PD patients with mild and moderate PD and age-matched controls, were examined by enzyme-linked immunosorbent assay (ELISA). Nonparametric statistics was used for data analysis. We found significantly elevated levels of a-synuclein autoantibodies in both plasma and CSF in mild PD compared to controls, followed by some decrease in moderate PD. Receiver operating characteristic and effect size analyses confirmed the diagnostic power of a-synuclein antibodies in both plasma and CSF. For the first time, we showed the correlation between plasma and CSF a-synuclein antibody levels for mild, moderate, and combined PD groups. This indicates the potentiality of a-synuclein antibodies as PD biomarker and the increased diagnostic power of their simultaneous analysis in plasma and CSF