State-of-the-art techniques for imaging the vascular microenvironment in craniofacial bone tissue engineering applications.
State-of-the-art techniques for imaging the vascular microenvironment in craniofacial bone tissue engineering applications.
复制标题
用于颅面骨组织工程应用中血管微环境成像的最先进技术。
DOI：
10.1152/ajpcell.00195.2022
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Pathak,ArvindP
中科院分区：
文献类型：
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作者：
Ren,Yunke;Senarathna,Janaka;Grayson,WarrenL;Pathak,ArvindP
血管化是通过生物工程构建或移植物再生肌肉骨骼组织的关键步骤。功能性血管系统为移植物微环境提供氧气和营养，促进伤口愈合，增强移植物与宿主组织的融合，并确保再生组织的长期存活。因此，成像新生血管（即血管生成）、微血管形态的变化以及移植物部位灌注的建立和维持（即血管微环境或VME）可以为移植物植入、伤口愈合提供必要的见解，并为组织工程（TE）结构的设计提供信息。在这篇综述中，我们将重点介绍在颅面TE应用中监测VME的最新成像方法，以及该领域的未来进展。我们描述了尖端的体内和体外成像方法如何产生关于VME参数的宝贵信息，这些参数可以帮助表征不同TE结构的有效性，并迭代地告知其设计以增强颅面骨再生。最后，我们阐述了新型TE结构、临床前模型系统、成像技术和系统生物学方法的整合如何引领一个“基于图像的组织工程”时代。
Vascularization is a crucial step during musculoskeletal tissue regeneration via bioengineered constructs or grafts. Functional vasculature provides oxygen and nutrients to the graft microenvironment, facilitates wound healing, enhances graft integration with host tissue, and ensures the long-term survival of regenerating tissue. Therefore, imaging de novo vascularization (i.e., angiogenesis), changes in microvascular morphology, and the establishment and maintenance of perfusion within the graft site (i.e., vascular microenvironment or VME) can provide essential insights into engraftment, wound healing, as well as inform the design of tissue engineering (TE) constructs. In this review, we focus on state-of-the-art imaging approaches for monitoring the VME in craniofacial TE applications, as well as future advances in this field. We describe how cutting-edge in vivo and ex vivo imaging methods can yield invaluable information regarding VME parameters that can help characterize the effectiveness of different TE constructs and iteratively inform their design for enhanced craniofacial bone regeneration. Finally, we explicate how the integration of novel TE constructs, preclinical model systems, imaging techniques, and systems biology approaches could usher in an era of “image-based tissue engineering.”