Adipose differentiation related protein (ADRP) expressed in transfected COS-7 cells selectively stimulates long chain fatty acid uptake
Adipose differentiation related protein (ADRP) expressed in transfected COS-7 cells selectively stimulates long chain fatty acid uptake
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DOI：
10.1074/jbc.274.24.16825
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发表时间：
1999-06-11
影响因子：
4.8
通讯作者：
Serrero, G
中科院分区：
文献类型：
--
作者：
Gao, J;Serrero, G
脂肪分化相关蛋白 (ADRP) 是一种从小鼠 1246 脂肪细胞 cDNA 文库中克隆的 50 kDa 新型蛋白，在脂肪细胞分化过程中快速诱导。我们检查了 ADRP 功能，并在此表明 ADRP 促进转染 ADRP cDNA 的 COS 细胞中的脂肪酸摄取。我们证明，与空载体转染的对照细胞相比，表达 ADRP 的 COS-7 细胞中长链脂肪酸的摄取以时间依赖性方式显着刺激。与对照细胞相比，表达 ADRP 的 COS-7 细胞中油酸摄取速度以剂量依赖性方式显着增加。在表达ADRP的细胞中，转运K-m为0.051μM，V-max为57.97pmol/10(5)细胞/分钟，在对照细胞中，K-m为0.093μM，V-max为20.13pmol/10(5)细胞/分钟。 4℃时测得的油酸摄取量仅为37℃时的10%，ADRP还刺激棕榈酸和花生四烯酸的摄取，但对中链脂肪酸如辛酸和葡萄糖的摄取没有影响。这些数据表明，ADRP 通过增加初始摄取速率来特异性增强长链脂肪酸的摄取，并提供有关 ADRP 作为长链脂肪酸的饱和转运成分的功能的新信息。
Adipose differentiation related protein (ADRP) is a 50-kDa novel protein cloned from a mouse 1246 adipocyte cDNA library, rapidly induced during adipocyte differentiation. We have examined ADRP function, and we show here that ADRP facilitates fatty acid uptake in COS cells transfected with ADRP cDNA We demonstrate that uptake of long chain fatty acids was significantly stimulated in a time-dependent fashion in ADRP-expressing COS-7 cells compared with empty vector-transfected control cells. Oleic acid uptake velocity increased significantly in a dose-dependent manner in ADRP-expressing COS-7 cells compared with control cells. The transport K-m was 0.051 mu M, and V-max was 57.97 pmol/10(5) cells/min in ADRP-expressing cells, and K-m was 0.093 mu M and V-max was 20.13 pmol/10(5) cells/min in control cells. The oleate uptake measured at 4 degrees C was only 10% that at 37 degrees C, ADRP also stimulated uptake of palmitate and arachidonate but had no effect on uptake of medium chain fatty acid such as octanoic acid and glucose. These data suggest that ADRP specifically enhances uptake of long chain fatty acids by increasing the initial rate of uptake and provide novel information about ADRP function as a saturable transport component for long chain fatty acids.