Study of the peak variance in isocratic and gradient liquid chromatography using the transport model.
Study of the peak variance in isocratic and gradient liquid chromatography using the transport model.
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DOI：
10.1016/j.chroma.2013.04.044
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发表时间：
2013-06
期刊：
影响因子：
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通讯作者：
Weiqiang Hao;Bin Di;Qiang Chen;Junde Wang;Yongbing Yang;Xiaoli Sun
中科院分区：
文献类型：
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作者：
Weiqiang Hao;Bin Di;Qiang Chen;Junde Wang;Yongbing Yang;Xiaoli Sun
从输运模型出发，推导了等规液相色谱和梯度液相色谱中的峰方差表达式。在数学处理中，考虑了停留时间，而没有指定溶剂强度的类型、梯度分布和板高度(H)随流动相组成(φ)的变化。通过应用坐标变换，利用拉普拉斯变换方法求解输运模型。得到了既适用于等度洗脱又适用于梯度洗脱的塔板高度方程。在此基础上，导出了阶梯梯度和线性梯度任意组合的平板高度方程。当溶剂强度为线性且H-φ图为抛物线时，这些方程将是代数的。给出了单阶梯、单线性和梯形梯度下的平板高度方程。
The expressions accounting for the peak variance in isocratic and gradient liquid chromatography is derived from the transport model. In mathematical treatments, the dwelling time is taken into account, and the type of solvent strength, gradient profile and the variation of plate height (H) with mobile phase composition (φ) is not specified. By applying a coordinate transformation, the transport model is solved by using the Laplace transform approach. A plate height equation that is suited for both isocratic and gradient elution is obtained. Based on this equation, the plate height equations for any combination of stepwise and linear gradients are derived. These equations will be algebraic when the solvent strength is linear and the H–φ plot is parabolic. The plate height equations for single stepwise, single linear and the ladder-like gradients are also given.