Optimisation of the analytical method for octa-, nona- and deca-brominated diphenyl ethers using gas chromatography-quadrupole mass spectrometry and isotope dilution
Optimisation of the analytical method for octa-, nona- and deca-brominated diphenyl ethers using gas chromatography-quadrupole mass spectrometry and isotope dilution
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DOI：
10.1080/03067319.2010.496040
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发表时间：
2011-01-01
影响因子：
2.6
通讯作者：
Tanabe, Shinsuke
中科院分区：
文献类型：
--
作者：
Eguchi, Akifumi;Isobe, Tomohiko;Tanabe, Shinsuke
使用配备电子轰击电离四极杆质谱仪 (GC-EI-qMS) 的气相色谱仪，优化了多溴二苯醚 (PBDE) 高溴同系物的分析方法以及生物和环境样品（贻贝、沉积物、灰尘）中的五种天然 PBDE 和三种 C-13(12) 标记同系物。在优化的仪器条件下，丰度和重复性随着离子源温度的升高而提高。 BDE-196、BDE-197、BDE-206、BDE-207 和 BDE-209 的仪器检测限 (IDL) 分别为 0.1、0.1、0.2、0.3 和 0.6 pg。与之前的报告相比，IDL 与电子捕获负电离 (ECNI) 或 EI 双聚焦扇形磁区 (EI-Sector) 质谱仪相同，表明使用传统 GC-EI-qMS 可以实现灵敏测定。通过分析参考材料和贻贝样品对该方法进行了验证。我们确认使用此方法量化的浓度在参考材料报告值的范围内。我们小组之前对贻贝进行了分析，发现了类似的浓度。因此，我们得出结论，传统的 GC-EI-qMS 可用于分析各种环境和生物基质中的高溴化 PBDE。
An analytical method for higher brominated congeners of polybrominated diphenyl ethers (PBDEs) was optimised using a gas chromatograph equipped with an electron impact ionisation-quadrupole mass spectrometer (GC-EI-qMS) and five native PBDEs and three C-13(12)-labelled congeners in biological and environmental samples (mussels, sediment, dust). In the optimised instrumental conditions, abundance and repeatability improved with increase in temperature of the ion source. The instrumental detection limits (IDLs) for BDE-196, BDE-197, BDE-206, BDE-207 and BDE-209 were 0.1, 0.1, 0.2, 0.3 and 0.6 pg, respectively. When compared to the previous reports, the IDLs were the same as for electron capture negative ionisation (ECNI) or EI-double focusing magnetic sector (EI-Sector) mass spectrometer, indicating that sensitive determination could be achieved using a conventional GC-EI-qMS. Validation of the method was carried out by the analysis of reference materials and mussel samples. We confirmed that the concentrations quantified using this method was in the range of reported values for reference materials. Similar concentrations were found in mussels, which were analysed previously by our group. Thus, we conclude that a conventional GC-EI-qMS can be applied for analysis of higher brominated PBDEs in various environmental and biota matrices.