REQUIREMENT FOR ACETATE OF A STREPTOMYCIN-RESISTANT STRAIN OF STAPHYLOCOCCUS-AUREUS
REQUIREMENT FOR ACETATE OF A STREPTOMYCIN-RESISTANT STRAIN OF STAPHYLOCOCCUS-AUREUS
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DOI：
10.1099/00221287-29-1-157
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发表时间：
1962-01-01
期刊：
影响因子：
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通讯作者：
LASCELLES, J
中科院分区：
文献类型：
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作者：
GARDNER, JF;LASCELLES, J
金黄色葡萄球菌的链霉素耐药菌株需氧生长需要氯化血红素，如果培养基中添加乙酸盐或丙酮酸盐，则在不含氯化血红素的情况下需氧或厌氧生长;尿嘧啶和嘌呤可增加这些有机酸的生长。亲本药物敏感菌株在无血红素的情况下需氧生长，但在厌氧生长时需要尿嘧啶或乙酸盐或丙酮酸盐。在两种菌株中，甲羟戊酸取代乙酸，活性约为乙酸的10倍。两种菌株的葡萄糖发酵产物没有明显差异，乳酸盐占主导地位（约占葡萄糖碳的85[degree]/o）;仅检测到少量乙酸盐。在需氧条件下，亲本菌株的悬浮液将葡萄糖氧化成乙酸盐，乙酸盐积累。突变株氧化葡萄糖乙酸只有当以前生长的氯化血红素或氯化血红素被添加到悬浮液的生物体。当有机体用乙酸盐代替氯化血红素生长时，乳酸盐是主要产物。突变体的能力，以形成足够的乙酸从葡萄糖用于生物合成的目的显然是依赖于一个功能性的电子传递链，涉及血蛋白。两种生物的提取物中均存在烟酰胺腺嘌呤二核苷酸连接的乳酸脱氢酶和丙酮酸氧化系统。这些酶在突变株的活性是相似的，无论是在氯化血红素或乙酸盐上生长的生物体。
A streptomycin-resistant strain of Staphylococcus aureus which requires haemin for aerobic growth grew either aerobically or anaero-bically in the absence of haemin provided the medium was supplemented with acetate or pyruvate; growth with these organic acids was increased by uracil and purines. The parent drug-sensitive strain grew aerobically without haemin but when grown anaerobically required either uracil or acetate or pyruvate. With both strains mevalonate replaced acetate and was about ten times more active. The products of glucose fermentation by both strains showed no gross difference, lactate being predominant (about 85[degree]/o of the glucose carbon); only small amounts of acetate were detected. Under aerobic conditions suspensions of the parent strain oxidized glucose to acetate which accumulated. The mutant strain oxidized glucose to acetate only when previously grown with haemin or when haemin was added to the suspension of organisms. When the organism was grown with acetate in place of haemin, lactate was the predominant product. The ability of the mutant to form sufficient acetate from glucose for biosynthetic purposes is apparently dependent on a functional electron transport chain involving haemoproteins. A nicotinamide-adenine dinucleotide-linked lactate dehydrogenase and a pyruvate oxidizing system are present in extracts of both organisms. The activity of these enzymes in the mutant strain was similar whether the organisms were grown on haemin or acetate.