Galanin expression is decreased by cAMP-elevating agents in cultured sympathetic ganglia.
Galanin expression is decreased by cAMP-elevating agents in cultured sympathetic ganglia.
复制标题
在培养的交感神经节中，cAMP 升高剂可降低甘丙肽的表达。
DOI：
10.1097/00001756-199904260-00012
复制
发表时间：
1999
期刊：
影响因子：
1.7
通讯作者：
Zigmond,RE
中科院分区：
文献类型：
--
作者：
Mohney,RP;Zigmond,RE
在多种条件下，在外周神经元中GALANIN表达与血管活性肠肽（VIP）的表达共调节。例如，成年大鼠上级颈神经节（SCG）切除后，两者的表达均增加。由于VIP参与调节其自身表达的正反馈回路，我们研究了VIP是否也增加甘丙肽表达。甘丙肽mRNA和肽在体内SCG中几乎检测不到，但在器官培养24-48 h后显著增加。VIP或毛喉素的培养基中加入减少甘丙肽mRNA的表达分别为75%和77%，并减少甘丙肽的表达分别为76%和82%，与对照培养基中培养的神经节。相反，异丙肾上腺素刺激并没有显着改变甘丙肽mRNA或肽的水平，与以前的观察结果，异丙肾上腺素发挥其对SCG非神经元细胞的作用，但不对神经元一致。结果表明，甘丙肽和VIP的差异调节交感神经元cAMP升高剂。
GALANIN expression is co-regulated in peripheral neurons with that of vasoactive intestinal peptide (VIP) under a variety of conditions. For example, the expression of both increase after explantation of adult rat superior cervical ganglia (SCG). Because VIP participates in a positive feedback loop regulating its own expression, we examined whether VIP also increases galanin expression. Galanin mRNA and peptide are nearly undetectable in the SCG in vivo, but increase dramatically after 24–48 h in organ culture. Addition of VIP or forskolin to the culture medium reduced galanin mRNA expression by 75% and 77%, respectively, and reduced galanin peptide expression by 76% and 82%, respectively, compared with ganglia cultured in control medium. In contrast, isoproterenol stimulation did not significantly alter levels of galanin mRNA or peptide, consistent with previous observations that isoproterenol exerts its effect on SCG non-neuronal cells, but not on neurons. The results indicate that galanin and VIP are differentially regulated in sympathetic neurons by cAMP-elevating agents.