Assessing the survival of exogenous plant microRNA in mice.
Assessing the survival of exogenous plant microRNA in mice.
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DOI：
10.1002/fsn3.113
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发表时间：
2014-07
影响因子：
3.9
通讯作者：
Xiao, ZhongDang
中科院分区：
文献类型：
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作者：
Liang, GaoFeng;Zhu, YanLiang;Sun, Bo;Shao, YouHua;Jing, AiHua;Wang, JunHua;Xiao, ZhongDang
MicroRNAs （miRNAs）是一类小RNA，是影响多种发育过程和调节RNA干扰（RNAi）的重要分子，在动物、植物和植物组织中大量存在，传统上以饮食为食。然而，植物小rna在动物饮食中的存活尚不清楚，来自膳食植物的mirna在动物胃肠道（GI）中的持久性仍存在争议。在本研究中，ICR小鼠被喂食从甘蓝中提取的植物总rna，剂量为10-50 μg。在RNA消耗后，收集小鼠的血清、粪便和各种组织，并分析几种mirna。外源植物mirna存在于动物的血清、粪便和组织中，这些外源植物mirna主要是通过口服获得的。在喂食含有MiR-172的植物RNA提取物的小鼠的胃、肠、血清和粪便中发现了甘蓝中富集程度最高的外源植物miRNA MiR-172。miR-172通过胃肠道的存活量因个体而异，在一个个体的胃中最多可恢复4.5%，在不同器官中的恢复范围为0.05-4.5%。此外，在小鼠的血液、脾脏、肝脏和肾脏中检测到miR-172。
MicroRNAs (miRNAs), a class of small RNAs, are important molecules that influence several developmental processes and regulate RNA interference (RNAi), and are abundant in animals, plants, and plant tissues that are traditionally consumed in the diet. The survival of plant small RNAs from the diet in animals, however, remains unclear, and the persistence of miRNAs from dietary plants in the animal gastrointestinal (GI) tract is still under debate. In this study, ICR mice were fed plant total RNAs in quantities of 10–50 μg, extracted from Brassica oleracea. Serum, feces, and various tissues were collected from the mice after RNA consumption and analyzed for several miRNAs. Exogenous plant miRNAs were present in the sera, feces, and tissues of animals and these exogenous plant miRNAs were primarily acquired orally. MiR-172, the most highly enriched exogenous plant miRNA in B. oleracea, was found in the stomach, intestine, serum, and feces of mice that were fed plant RNA extracts including miR-172. The amount of miR-172 that survived passage through the GI tract varied among individuals, with a maximum of 4.5% recovered at the stomach of one individual, and had a range of 0.05–4.5% in different organs. Furthermore, miR-172 was detected in the blood, spleen, liver, and kidney of mice.