BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling
BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling
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DOI：
10.1016/s0092-8674(02)00812-7
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发表时间：
2002-07-26
期刊：
影响因子：
64.5
通讯作者：
Walker, JC
中科院分区：
文献类型：
--
作者：
Li, J;Wen, JQ;Walker, JC
油菜素类固醇通过蛋白质复合物调节植物生长和发育，该蛋白质复合物包括富含亮氨酸的重复受体样蛋白激酶 (LRR-RLK) 油菜素类固醇不敏感 1 (BRI1)。激活标签用于识别 bri1 的显性遗传抑制因子 bak1-1D（bri1 相关受体激酶 1-1 显性），它编码与 BRI1 不同的 LRR-RLK。 BAK1 的过度表达会导致器官表型延长，而 BAK1 的无效等位基因则显示出半矮化表型，并且对油菜素类固醇 (BR) 的敏感性降低。 BAK1 是一种丝氨酸/苏氨酸蛋白激酶，BRI1 和 BAK1 在体外和体内相互作用。 BAK1 显性失活突变等位基因的表达会导致严重的矮化表型，类似于无效 bri1 等位基因的表型。这些结果表明 BAK1 是 BR 信号传导的一个组成部分。
Brassinosteroids regulate plant growth and development through a protein complex that includes the leucine-rich repeat receptor-like protein kinase (LRR-RLK) brassinosteroid-insensitive 1 (BRI1). Activation tagging was used to identify a dominant genetic suppressor of bri1, bak1-1D (bri1-associated receptor kinase 1-1Dominant), which encodes an LRR-RLK, distinct from BRI1. Overexpression of BAK1 results in elongated organ phenotypes, while a null allele of BAK1 displays a semidwarfed phenotype and has reduced sensitivity to brassinosteroids (BRs). BAK1 is a serine/threonine protein kinase, and BRI1 and BAK1 interact in vitro and in vivo. Expression of a dominant-negative mutant allele of BAK1 causes a severe dwarf phenotype, resembling the phenotype of null bri1 alleles. These results indicate BAK1 is a component of BR signaling.