Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia
Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia
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DOI：
10.1128/jvi.79.2.884-895.2005
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发表时间：
2005-01-01
影响因子：
5.4
通讯作者：
Yuen, KY
中科院分区：
文献类型：
--
作者：
Woo, PCY;Lau, SKP;Yuen, KY
尽管对呼吸道感染患者进行了广泛的实验室调查，但在相当大比例的患者中无法确定任何微生物原因。在过去的3年里，发现了几种新的呼吸道病毒，包括人类偏肺病毒、严重急性呼吸综合征(SARS)冠状病毒(SARSCoV)和人类冠状病毒NL63。在此，我们报告了从深圳回来的71岁肺炎患者中国身上发现的另一种新型冠状病毒-冠状病毒香港1型(CoV-HKU1)。定量逆转录-聚合酶链式反应显示，在发病第一周，患者鼻咽抽吸物中的CoV-HKU1 RNA含量为8.5-9.6×10(6)拷贝/毫升，随后几周逐渐下降至检测不到的水平。他的血清中针对CoV-HKU1重组核衣壳蛋白的特异性抗体水平不断上升，免疫球蛋白M(IgM)滴度分别为1：20、1：40和1：80，免疫球蛋白G滴度为
Despite extensive laboratory investigations in patients with respiratory tract infections, no microbiological cause can be identified in a significant proportion of patients. In the past 3 years, several novel respiratory viruses, including human metapneumovirus, severe acute respiratory syndrome (SARS) coronavirus (SARSCoV), and human coronavirus NL63, were discovered. Here we report the discovery of another novel coronavirus, coronavirus HKU1 (CoV-HKU1), from a 71-year-old man with pneumonia who had just returned from Shenzhen, China. Quantitative reverse transcription-PCR showed that the amount of CoV-HKU1 RNA was 8.5 to 9.6 X 10(6) copies per ml in his nasopharyngeal aspirates (NPAs) during the first week of the illness and dropped progressively to undetectable levels in subsequent weeks. He developed increasing serum levels of specific antibodies against the recombinant nucleocapsid protein of CoV-HKU1, with immunoglobulin M (IgM) titers of 1:20, 1:40, and 1:80 and IgG titers of