Free radical scavenging capacity and protective effect of Bacopa monniera L. on DNA damage
Free radical scavenging capacity and protective effect of Bacopa monniera L. on DNA damage
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DOI：
10.1002/ptr.1061
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发表时间：
2003-09
影响因子：
7.2
通讯作者：
A. Russo;A. Izzo;F. Borrelli;M. Renis;A. Vanella
中科院分区：
文献类型：
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作者：
A. Russo;A. Izzo;F. Borrelli;M. Renis;A. Vanella
Bacopa monniera L.（玄参科）(BM) 是一种阿育吠陀药物，临床上用于增强记忆、癫痫、失眠以及作为温和的镇静剂。在这项工作中，研究了 BM 甲醇提取物的自由基清除能力以及对 H2O2 紫外光解诱导的 DNA 裂解的影响。此外，我们还研究了这种植物提取物是否能够减少过氧化氢诱导的人类非永生化成纤维细胞的细胞毒性和 DNA 损伤。它表现出剂量依赖性的自由基清除能力和对 DNA 裂解的保护作用。这些结果得到了对 H2O2 诱导的人类非永生化成纤维细胞的细胞毒性和 DNA 损伤的显着保护作用的证实。 BM 的抗氧化能力至少可以部分解释其在实验动物和临床情况下产生的抗应激、免疫调节、认知促进、抗炎和抗衰老作用，并可能证明对其其他有益特性的进一步研究是合理的。此外，该实验证据表明，由于其抗氧化活性，这种阿育吠陀药物可能可用于治疗自由基产生发挥关键作用的人类病理。版权所有 © 2003 约翰·威利父子有限公司
Bacopa monniera L. (family Scrophulariaceae) (BM) is an Ayurvedic medicine, clinically used for memory enhancing, epilepsy, insomnia and as a mild sedative. In this work, the free radical scavenging capacity of a methanol extract of BM and the effect on DNA cleavage induced by H2O2 UV‐photolysis was investigated. In addition, we examined whether this plant extract is capable of reducing the hydrogen peroxide‐induced cytotoxicity and DNA damage in human non‐immortalized fibroblasts. It showed a dose‐dependent free radical scavenging capacity and a protective effect on DNA cleavage. These results were confirmed by a significant protective effect on H2O2 – induced cytoxicity and DNA damage in human non‐immortalized fibroblasts. The antioxidant capacity of BM may explain, at least in part, the reported antistress, immunomodulatory, cognition‐facilitating, antiinflammatory and antiaging effects produced by it in experimental animals and in clinical situations and may justify further investigation of its other beneficial properties. Moreover, this experimental evidence suggests that because of its antioxidant activity, this Ayurvedic drug may be useful in the treatment of human pathologies in which free radical production plays a key role. Copyright © 2003 John Wiley & Sons, Ltd.