Development of a cDNA array for chicken gene expression analysis
Development of a cDNA array for chicken gene expression analysis
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DOI：
10.1186/1471-2164-6-13
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发表时间：
2005-02-04
期刊：
影响因子：
4.4
通讯作者：
Delrow, J
中科院分区：
文献类型：
--
作者：
Burnside, J;Neiman, P;Delrow, J
背景：由于缺乏包含大量基因的芯片，微阵列技术在鸡功能基因组分析中的应用一直受到限制。结果：我们利用BBSRC、特拉华大学和弗雷德·哈钦森癌症研究中心收集的鸡EST序列制备了cDNA阵列。从代表24个不同成人或胚胎组织的总共363,838个鸡EST中，选择了一组11,447个非冗余EST，并将其添加到现有的免疫组织克隆集(4,162个)和鸡法氏囊细胞系(DT40)中。质控分析表明，阵列上有13,007个可用特征，包括160个控制点。该芯片提供了在多种组织中表达的mRNAs的广泛覆盖；此外，还可以检测到各种组织特有表达的克隆。结论：具有13,007个特征的鸡多组织基因芯片现已提供给学术研究人员，网址为http：//genology@fhcrc。Org.该阵列上所有特征的序列信息都在GenBank中，可以很容易地获得克隆。目标用户包括比较和发育生物学、免疫学、疫苗和农业技术方面的研究人员。这些阵列将成为整个以鸡为模型的研究界的重要资源。
Background: The application of microarray technology to functional genomic analysis in the chicken has been limited by the lack of arrays containing large numbers of genes.Results: We have produced cDNA arrays using chicken EST collections generated by BBSRC, University of Delaware and the Fred Hutchinson Cancer Research Center. From a total of 363,838 chicken ESTs representing 24 different adult or embryonic tissues, a set of 11,447 non- redundant ESTs were selected and added to an existing collection of clones ( 4,162) from immune tissues and a chicken bursal cell line ( DT40). Quality control analysis indicates there are 13,007 useable features on the array, including 160 control spots. The array provides broad coverage of mRNAs expressed in many tissues; in addition, clones with expression unique to various tissues can be detected.Conclusions: A chicken multi- tissue cDNA microarray with 13,007 features is now available to academic researchers from http:// genomics@ fhcrc. org. Sequence information for all features on the array is in GenBank, and clones can be readily obtained. Targeted users include researchers in comparative and developmental biology, immunology, vaccine and agricultural technology. These arrays will be an important resource for the entire research community using the chicken as a model.