Gene expression analysis of Salmonella enterica Enteritidis Nal(R) and Salmonella enterica Kentucky 3795 exposed to HCl and acetic acid in rich medium.
Gene expression analysis of Salmonella enterica Enteritidis Nal(R) and Salmonella enterica Kentucky 3795 exposed to HCl and acetic acid in rich medium.
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DOI：
10.1089/fpd.2011.0984
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发表时间：
2012-03
影响因子：
2.8
通讯作者：
R. Joerger;C. Sartori;J. Frye;J. B. Turpin;C. Schmidt;Michael McClelland;S. Porwollik
中科院分区：
文献类型：
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作者：
R. Joerger;C. Sartori;J. Frye;J. B. Turpin;C. Schmidt;Michael McClelland;S. Porwollik
在美国，肯塔基血清型已成为最常从鸡中分离出的肠沙门氏菌血清型之一。这种流行的原因尚不清楚。属于不同血清型的家禽沙门氏菌分离株的表型比较表明，肯塔基州分离株对酸的反应与大多数其他血清型不同。对充气指数生长家禽分离株、肠沙门氏菌肯塔基 3795 和肠炎 Nal(R) 进行微阵列和 qPCR 分析，将其暴露于用 HCl 调节至 pH 4.5 的胰蛋白酶大豆肉汤 (TSB) 中 10 分钟，并用 HCl 或乙酸调节至 pH 5.5。通过微阵列分析获得的数据表明，在酸性条件下，与肠炎Nal相比，Kentucky 3795菌株中上调或下调的基因更多。酸暴露通常会导致能量代谢基因上调和蛋白质合成基因（尤其是核糖体蛋白质基因）下调。两种菌株似乎都类似地利用基于赖氨酸的 pH 稳态系统，因为在酸性条件下观察到 cadB 的上调。已知参与酸反应的调节基因（rpoS、fur、phoPQ）的表达在两个分离株中显示出相似的趋势。观察到 Kentucky 3795 和 Enteritidis Nal(R) 在 hdeB 样基因座 SEN1493（可能编码对酸反应重要的分子伴侣）表达方面存在差异，并且注意到其他基因（例如涉及柠檬酸盐利用和运动的基因）表达方面的一些差异。分离株Kentucky 3795和肠炎Nal(R)对酸的转录反应的早期阶段似乎是相似的，但在反应的范围和某些方面存在差异。观察到的数量差异可能会导致蛋白质水平的差异，从而解释观察到的肯塔基血清型和其他沙门氏菌血清型的酸性表型差异。
In the United States, serovar Kentucky has become one of the most frequently isolated Salmonella enterica serovars from chickens. The reasons for this prevalence are not well understood. Phenotypic comparisons of poultry Salmonella isolates belonging to various serovars demonstrated that serovar Kentucky isolates differed from those of most other serovars in their response to acid. Microarray and qPCR analyses were performed with aerated exponentially growing poultry isolates, Salmonella enterica serovar Kentucky 3795 and Enteritidis Nal(R), exposed for 10 min to tryptic soy broth (TSB) adjusted to pH 4.5 with HCl and to pH 5.5 with HCl or acetic acid. Data obtained by microarray analysis indicated that more genes were up- or down-regulated in strain Kentucky 3795 than in Enteritidis Nal(R) under acidic conditions. Acid exposure in general caused up-regulation of energy metabolism genes and down-regulation of protein synthesis genes, particularly of ribosomal protein genes. Both strains appear to similarly utilize the lysine-based pH homeostasis system, as up-regulation of cadB was observed under the acidic conditions. Expression of regulatory genes (rpoS, fur, phoPQ) known to be involved in the acid response showed similar trends in both isolates. Differences between Kentucky 3795 and Enteritidis Nal(R) were observed with respect to the expression of the hdeB-like locus SEN1493 (potentially encoding a chaperone important to acid response), and some differences in the expression of other genes such as those involved in citrate utilization and motility were noted. It appears that the early stages of the transcriptional response to acid by isolates Kentucky 3795 and Enteritidis Nal(R) are similar, but differences exist in the scope and in some facets of the response. Possibly, the quantitative differences observed might lead to differences in protein levels that could explain the observed differences in the acid phenotype of serovar Kentucky and other Salmonella serovars.