A reversible near-infrared fluorescence probe for reactive oxygen species based on Te-rhodamine
A reversible near-infrared fluorescence probe for reactive oxygen species based on Te-rhodamine
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DOI：
10.1039/c2cc18011a
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发表时间：
2012-01-01
影响因子：
4.9
通讯作者：
Nagano, Tetsuo
中科院分区：
文献类型：
--
作者：
Koide, Yuichiro;Kawaguchi, Mitsuyasu;Nagano, Tetsuo
利用碲（Te）原子的氧化还原性质，设计并合成了一种活性氧（ROS）的可逆近红外荧光探针2-Me TeR。2-Me TeR被各种ROS氧化为荧光2-Me TeOR，而产生的2-Me TeOR在谷胱甘肽存在下快速还原以再生2-Me TeR。这种氧化还原诱导的2-Me TeR的可逆NIR荧光响应使我们能够检测过氧化氢刺激的HL-60细胞中ROS的内源性产生和随后的细胞内还原环境的稳态恢复。该探针有望用于连续监测体内ROS产生的动态。
We have designed and synthesized a reversible near-infrared (NIR) fluorescence probe, 2-Me TeR, for reactive oxygen species (ROS), utilizing the redox properties of the tellurium (Te) atom. 2-Me TeR is oxidized to fluorescent 2-Me TeOR by various ROS, while the generated 2-Me TeOR is quickly reduced in the presence of glutathione to regenerate 2-Me TeR. This redox-induced reversible NIR-fluorescence response of 2-Me TeR allowed us to detect the endogenous production of ROS and subsequent homeostatic recovery of the intracellular reductive environment in hydrogen peroxide-stimulated HL-60 cells. This probe is expected to be useful for monitoring the dynamics of ROS production continuously in vivo.