Gelatin Hydrogel Prepared by Photo-initiated Polymerization and Loaded with TGF-β1 for Cartilage Tissue Engineering
Gelatin Hydrogel Prepared by Photo-initiated Polymerization and Loaded with TGF-β1 for Cartilage Tissue Engineering
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DOI：
10.1002/mabi.200900275
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发表时间：
2009-12-08
影响因子：
4.6
通讯作者：
Gao, Changyou
中科院分区：
文献类型：
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作者：
Hu, Xiaohong;Ma, Lie;Gao, Changyou
明胶是一种天然来源的蛋白质，具有良好的细胞相容性，广泛应用于组织工程，特别是以水凝胶的形式。然而，为了获得具有足够好的机械性能的水凝胶，仍然需要采取措施。本工作利用甲基丙烯酸（MA）对明胶分子进行修饰，得到可交联明胶（GM），通过光引发聚合形成化学交联水凝胶。胶凝时间可以很容易地调节，并且与 GM 浓度呈反比关系。光照射20分钟后，高分辨率魔角旋转核磁共振波谱（1H HR-MAS NMR）的氢谱中没有检测到双碳键。随着GM浓度的增加，水凝胶的储能模量和损耗模量增加，但溶胀率和网格尺寸减小。水凝胶的重量损失也受到聚合物浓度的影响。将转化生长因子-β1（TGF-β1）掺入转基因水凝胶中以提高其生物活性。体外软骨细胞培养表明，GM水凝胶确实具有良好的支持软骨细胞生长和维持软骨细胞表型的性能。 TGF-β1的掺入可以进一步提高细胞增殖和细胞外基质分泌方面的生物活性。
Gelatin is a nature‐derived protein having good cytocompatibility, and widely used in tissue engineering particularly in a form of a hydrogel. To obtain the hydrogel with good enough mechanical properties, however, measures are still need to be taken. In this work, the gelatin molecule was modified with methacrylic acid (MA) to obtain crosslinkable gelatin (GM), which formed a chemically crosslinked hydrogel by photoinitiating polymerization. The gelation time could be easily tuned and showed an inverse relationship with the GM concentration. After photo‐irradiation for 20 min there was no detectable double carbon bond in the hydrogen spectrum of high resolution magic angle spinning nuclear magnetic resonance spectroscopy (1H HR‐MAS NMR). With the increase of the GM concentration, storage modulus and loss modulus of the hydrogels increased, but their swelling ratio and mesh size decreased. Weight loss of the hydrogels was also affected by the polymer concentration. Transform growth factor‐β1 (TGF‐β1) was incorporated into the GM hydrogel to improve its bioactivity. In vitro chondrocyte culture showed that the GM hydrogel had indeed good performance to support chondrocyte growth and maintain chondrocytic phenotype. Incorporation of TGF‐β1 could further improve the biological activity in terms of cell proliferation and extracellular matrix secretion.