Metallic Element Chelated Tag Labeling (MeCTL) for Quantitation of N-Glycans in MALDI-MS
Metallic Element Chelated Tag Labeling (MeCTL) for Quantitation of N-Glycans in MALDI-MS
复制标题
用于 MALDI-MS 中 N-聚糖定量的金属元素螯合标签标记 (MeCTL)
DOI：
10.1021/acs.analchem.7b01051
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发表时间：
2017
影响因子：
7.4
通讯作者：
Lu Haojie
中科院分区：
文献类型：
--
作者：
Yang Lijun;Peng Ye;Jiao Jing;Tao Tao;Yao Jun;Zhang Ying;Lu Haojie
N-糖基化在主要的生物学和病理学过程中起着重要的作用。定量N-聚糖是重要的，因为聚糖改变与许多疾病有关。在这项研究中，我们开发了一种新的N-聚糖定量方法，通过还原胺化金属元素螯合标签标记（MeCTL）。该方法标记效率高，定量准确，重复性好（CV < 17.03%），线性良好（R2> 0.99），动态范围在2个数量级以内。此外，它提供了显著的交叉环片段化以区分N-聚糖异构体。此外，可以通过与几种不同的稀土元素螯合实现多重定量。最后，该策略已成功用于评估与CRC相关的人血清中的N-聚糖变化，表明其在临床应用中的潜力，包括疾病N-糖组谱和相对定量。
N-glycosylation plays an important role in chief biological and pathological processes. Quantifying the N-glycan is important since glycan alterations are related to many diseases. In this study, we developed a novel N-glycan quantitation approach using metallic element chelated tag labeling (MeCTL) through reductive amination. The MeCTL strategy is of high labeling efficiency and accurate in quantitation with high reproducibility (CV < 17.03%) and good linearity (R2> 0.99) within 2 orders of magnitude of dynamic range. Additionally, it provides significant cross-ring fragmentation to distinguish N-glycan isomers. Furthermore, multiplex quantitation by chelation with several different rare earth elements can be achieved. At last, this strategy has been successfully used for evaluation of N-glycan changes in human serum associated with CRC, indicating its potential in clinical applications including disease N-glycome profiling and relative quantitation.