Simple SNP typing assay using a base-discriminating fluorescent probe
Simple SNP typing assay using a base-discriminating fluorescent probe
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DOI：
10.1039/b515923g
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发表时间：
2006-02-01
影响因子：
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通讯作者：
Saito, I
中科院分区：
文献类型：
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作者：
Okamoto, A;Tainaka, K;Saito, I
我们已经开发了一个新的概念，涉及单核苷酸多态性（SNP）分型的单步均相方法。在该方法中，将含有碱基识别荧光（BDF）碱基的探针加入到样品溶液中。含BDF碱基的DNA通常仅显示弱荧光，但当其识别杂交链中特定位点处的靶碱基时发射强蓝色荧光。通过利用该特征，实现了简单的混合-读取SNP分型测定，而无需任何繁琐的探针设计或洗涤过程以排除杂交错误或任何添加的DNA修饰酶。这与传统方法有很大不同。我们使用BDF检测试剂盒轻松、高准确度地同时分析了多个样品。
We have developed a new concept involving a single-step homogeneous method for single-nucleotide polymorphism (SNP) typing. in this method, a probe containing base-discriminating fluorescent (BDF) bases is added to a sample solution. BDF base-containing DNA usually shows only a weak fluorescence, but emits a strong blue fluorescence when it recognizes a target base at a specific site in a hybridized strand. By utilizing this feature, a simple mix-and-read SNP typing assay was achieved without any tedious probe-designing or washing processes for exclusion of hybridization error or any addition of DNA-modifying enzymes. This is very different from conventional methods. We simultaneously analyzed a number of samples with ease, with a high accuracy, using our BDF assay.