Use of enriched stable isotopes to determine zinc and iron absorption in elderly men.
Use of enriched stable isotopes to determine zinc and iron absorption in elderly men.
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使用浓缩稳定同位素测定老年男性的锌和铁吸收量。
DOI：
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发表时间：
1982
影响因子：
7.1
通讯作者：
S. Margen
中科院分区：
文献类型：
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作者：
J. Turnlund;M. Michel;W. R. Keyes;J. King;S. Margen
使用浓缩稳定同位素 70Zn 和 58Fe 测定了 7 名老年男性对锌和铁的吸收情况。通过离子交换从粪便样品中分离出锌并通过溶剂萃取从粪便样品中分离出铁后，使用热电离磁扇形质谱法对锌和铁的氯化物溶液进行同位素比分析。当每天喂食含有 15 毫克锌和 10 毫克铁的半纯化饮食时，七名受试者的平均表观锌吸收率为 17.3 +/- 3.1%（平均值 +/- SEM），平均铁吸收率为 7.9 +/- 2.5%（平均值 +/- SEM）。吸收量相当于平均每天 2.6 毫克锌和 0.8 毫克铁。这些平均锌和铁值接近于之前对锌和铁内源损失的估计。
The absorption of zinc and iron was determined in seven elderly men using the enriched stable isotopes 70Zn and 58Fe. Analyses of isotopic ratios were done using thermal ionization magnetic sector mass spectrometry on chloride solutions of zinc and of iron after separation of zinc from fecal samples by ion exchange and of iron by solvent extraction. Mean apparent zinc absorption in the seven subjects was 17.3 +/- 3.1% (mean +/- SEM) and mean iron absorption was 7.9 +/- 2.5% (mean +/- SEM) when fed a semipurified diet containing 15 mg of zinc and 10 mg of iron daily. Quantities absorbed were equivalent to an average of 2.6 mg of zinc and 0.8 mg of iron per day. These average zinc and iron values are close to previous estimates of endogenous losses of zinc and iron.