The IL-2 receptor present on human embryonic fibroblasts is functional in the absence of P64/IL-2R gamma chain.
The IL-2 receptor present on human embryonic fibroblasts is functional in the absence of P64/IL-2R gamma chain.
复制标题
人胚胎成纤维细胞上存在的 IL-2 受体在缺乏 P64/IL-2R γ 链的情况下仍具有功能。
作者：
S. Piaisance;E. Rubinstein;A. Alileche;P. Benolt;C. Jasmin;B. Azzarone
在这项研究中，我们研究了IL-2 R γ基因表达在几个人胚胎成纤维细胞表达IL-2受体（IL-2 R）的其他成分。聚合酶链反应不允许我们在这些细胞中检测IL-2 R γ转录物，但IL-2 R α β的功能不受影响。事实上，在人IL-2 R α + β + γ-胚胎肺成纤维细胞ICIG-7中，IL-2诱导了与先前在淋巴细胞中报道的相同的现象：IL-2 R α β-IL-2复合物的快速内化、细胞蛋白质（56和38 kDa）的特异性磷酸化和ICAM-1表达的上调。IL-2诱导ICAM-1仅在稀疏培养物和IL-2浓度超过180 pM时观察到。我们还观察到，在这些成纤维细胞，上调ICAM-2表达的IL-2，在稀疏和密集的文化。这些数据表明，p64/IL-2 R γ在人胚胎成纤维细胞中的表达与IL-2 R传递生物信号的能力无关。
In this study, we have investigated the IL-2R gamma gene expression in several human embryonic fibroblasts which express other components of the IL-2 receptor (IL-2R). Polymerase chain reaction did not allow us to detect IL-2R gamma transcripts in these cells but the functionality of the IL-2R alpha beta was not affected. Indeed, in the human IL-2R alpha+beta+gamma- embryonic lung fibroblasts ICIG-7, IL-2 induced identical phenomena to those previously reported in lymphoid cells: rapid internalization of the IL-2R alpha beta-IL-2 complex, specific phosphorylation of cellular proteins (56 and 38 kDa) and up-regulation of ICAM-1 expression. IL-2 induction of ICAM-1 was only observed in sparse cultures and for IL-2 concentrations over 180 pM. We have also observed, in these fibroblastic cells, the up-regulation of ICAM-2 expression by IL-2, both in sparse and dense cultures. These data show that p64/IL-2R gamma expression in human embryonic fibroblasts does not correlate with the ability of the IL-2R to deliver a biological signal.