In vivo Imaging of Mitochondrial Transport in Single-Axon Regeneration of Zebrafish Mauthner Cells.
In vivo Imaging of Mitochondrial Transport in Single-Axon Regeneration of Zebrafish Mauthner Cells.
复制标题
斑马鱼 Mauthner 细胞单轴突再生中线粒体运输的体内成像。
DOI：
10.3389/fncel.2017.00004
复制
发表时间：
2017
影响因子：
5.3
通讯作者：
Hu B
中科院分区：
文献类型：
--
作者：
Xu Y;Chen M;Hu B;Huang R;Hu B
线粒体运输对于神经元功能至关重要，但线粒体运输与活体脊椎动物中枢神经系统（CNS）中轴突再生之间联系的证据仍然有限。在这里，我们开发了一种新模型，通过非侵入性体内成像探索斑马鱼单个毛特纳轴突（M轴突）中的线粒体运输。为了证实使用该模型的可行性，我们用诺考达唑处理标记的斑马鱼，并证明它可以破坏线粒体运输。我们还使用双光子激光轴突切除术对M轴突进行精确切断，并同时记录它们的再生和活体斑马鱼幼虫线粒体运输的过程。研究结果表明，具有更强再生能力的受损轴突能够维持更大的线粒体运动。此外，为了刺激轴突再生，二丁酰环腺苷单磷酸 (db-cAMP) 治疗也可以增强线粒体运动。总而言之，我们的结果提供了新的证据，证明线粒体运动与活脊椎动物中枢神经系统中的轴突再生呈正相关。这个有前景的模型将有助于利用各种遗传和药理学技术进一步研究轴突再生和线粒体动力学之间的相互作用。
Mitochondrial transport is essential for neuronal function, but the evidence of connections between mitochondrial transport and axon regeneration in the central nervous system (CNS) of living vertebrates remains limited. Here, we developed a novel model to explore mitochondrial transport in a single Mauthner axon (M axon) of zebrafish with non-invasive in vivo imaging. To confirm the feasibility of using this model, we treated labeled zebrafish with nocodazole and demonstrated that it could disrupt mitochondrial transport. We also used two-photon laser axotomy to precisely axotomize M axons and simultaneously recorded their regeneration and the process of mitochondrial transport in living zebrafish larvae. The findings showed that the injured axons with stronger regenerative capability maintain greater mitochondrial motility. Furthermore, to stimulate axon regeneration, treatment with dibutyryl cyclic adenosine monophosphate (db-cAMP) could also augment mitochondrial motility. Taken together, our results provide new evidence that mitochondrial motility is positively correlated with axon regeneration in the living vertebrate CNS. This promising model will be useful for further studies on the interaction between axon regeneration and mitochondrial dynamics, using various genetic and pharmacological techniques.