IGF-1 and BMP-2 Induces Differentiation of Adipose-Derived Mesenchymal Stem Cells into Chondrocytes-Like Cells
IGF-1 and BMP-2 Induces Differentiation of Adipose-Derived Mesenchymal Stem Cells into Chondrocytes-Like Cells
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DOI：
10.1007/s10439-009-9892-x
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发表时间：
2010-04-01
影响因子：
3.8
通讯作者：
Li, Jianjun
中科院分区：
文献类型：
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作者：
An, Chunhou;Cheng, Yang;Li, Jianjun
关节软骨缺损是常见的，导致显著的发病率。利用多能干细胞的组织工程是一种新的有前途的软骨修复模式。在本研究中，我们研究了兔脂肪干细胞（ADSCs）的软骨形成。我们分离了兔ADSCs，并用编码人胰岛素生长样因子1（IGF-1）和骨形态发生蛋白2（BMP-2）的构建体转染这些细胞。我们研究了这些转染细胞的生长和形态以及它们的II型胶原和MMP-3的产生。我们发现IGF-1和BMP-2驱动ADSCs的软骨形成，其显示成熟的软骨细胞样细胞并形成软骨结节。这些细胞还产生II型胶原，MMP-3的产生减少。我们的研究结果表明，人ADSCs可以分化为软骨细胞样细胞由IGF-1和BMP-2驱动，并举行作为一个丰富的和现成的干细胞来源的软骨修复和再生的承诺。
Articular cartilage defects are common, causing significant morbidities. Tissue engineering using pluripotent stem cells is a new promising modality for cartilage repair. In the current study, we investigated the chondrogenesis of rabbit adipose-derived stem cells (ADSCs). We isolated rabbit ADSCs and transfected these cells with constructs encoding human insulin growth like factor 1 (IGF-1) and bone morphogenic protein 2 (BMP-2). We examined the growth and morphology of these transfected cells and their production of type II collagen and MMP-3. We found that IGF-1 and BMP-2 drove the chondrogenesis of ADSCs, which showed mature chondrocyte-like cells and formed cartilage nodules. These cells also produced type II collagen with a reduced production of MMP-3. Our findings suggested that human ADSCs could differentiate into chondrocyte-like cells driven by IGF-1 and BMP-2 and held promises as an abundant and ready source of stem cells for cartilage repair and regeneration.