Expression of mouse protamine 1 genes in transgenic mice.
Expression of mouse protamine 1 genes in transgenic mice.
复制标题
转基因小鼠中小鼠鱼精蛋白 1 基因的表达。
DOI：
10.1111/j.1749-6632.1989.tb25897.x
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发表时间：
1989
影响因子：
5.2
通讯作者：
Brinster,RL
中科院分区：
文献类型：
--
作者：
Peschon,JJ;Behringer,RR;Palmiter,RD;Brinster,RL
小鼠精蛋白基因仅在精细胞中表达。小鼠精蛋白1 （mP1）转录调控元件可以靶向标记的mP1转基因基因或mP1嵌合基因在转基因小鼠精细胞中的表达。相对于转录起始位点-40 -465 bp之间的序列是精子表达所必需的，而翻译起始点的3'序列是可缺性的。mP1转录调控序列用于指导有毒基因产物在精子中的表达。描述了精细胞中毒素表达的表型后果。
Mouse protamine genes are expressed exclusively in spermatids. Mouse protamine 1 (mP1) transcriptional regulatory elements can target the expression of either marked mP1 transgenes or mP1 chimeric genes to spermatids in transgenic mice. Sequences between-40 and-465 bp relative to the transcription start site are required for expression in spermatids, whereas sequences 3'of the point of translation initiation are dispensable. mP1 transcriptional regulatory sequences were used to direct the expression of a toxic gene product to spermatids. The phenotypic consequences of toxin expression in spermatids are described.