De novo biosynthesis of linoleic acid and its conversion to the hydrocarbon (Z,Z)-6,9-heptadecadiene in the astigmatid mote, Carpoglyphus lactis: Incorporation experiments with 13C-labeled glucose
De novo biosynthesis of linoleic acid and its conversion to the hydrocarbon (Z,Z)-6,9-heptadecadiene in the astigmatid mote, Carpoglyphus lactis: Incorporation experiments with 13C-labeled glucose
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亚油酸的从头生物合成及其在无光微粒中转化为碳氢化合物 (Z,Z)-6,9-十七碳二烯：13C 标记葡萄糖的掺入实验
DOI：
10.1016/j.ibmb.2013.11.006
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Kuwahara Y.
中科院分区：
文献类型：
--
作者：
Shimizu N;Naito M;Mori N;Kuwahara Y.
研究了乳炭螨亚油酸(LA)的新生物合成及其向(Z，Z)-6，9-十七碳二烯的转化。使用[1-13C]-d-葡萄糖进行13C给药的实验表明，13C原子被结合到从螨虫中提取的总脂的LA中，导致所有偶数碳的标记。这一结果表明LA是由13C标记的乙酰辅酶A产生的，这是直接新生物合成的标志。在这些涉及[1-13C]-d-葡萄糖的喂食实验中，13C原子也被引入到(Z，Z)-6，9-十七二烯中，这是该螨的主要分泌成分之一。(Z，Z)-6，9-十七二烯在奇数碳上的标记模式与失去羧基碳后的LA的标记模式一致。结果表明，在不需要这种必需脂肪酸的情况下，螨类可以稳定地将LA转化为(Z，Z)-6，9-十七碳二烯。
De novobiosynthesis of linoleic acid (LA) and its conversion to (Z,Z)-6,9-heptadecadiene were examined inCarpoglyphus lactis(Acarina, Carpoglyphidae). Experiments involving13C-administration using [1-13C]-d-glucose revealed that13C atoms were incorporated into LA of total lipid extracted from the mite, resulting in labeling of all even-numbered carbons. This result demonstrated that LA was produced from13C-labeled acetyl-CoA, which is indicative of directde novobiosynthesis. In these feeding experiments involving [1-13C]-d-glucose,13C atoms were also incorporated into (Z,Z)-6,9-heptadecadiene, which is one of the major secretory components in the mite. The labeling pattern of (Z,Z)-6,9-heptadecadiene at odd-numbered carbons agreed well with that of LA after loss of the carboxyl carbon. It was concluded that the mites could stably convert LA into (Z,Z)-6,9-heptadecadiene without the dietary requirement of this essential fatty acid.