Biodegradation of phenol by Candida tropicalis sp.: Kinetics, identification of putative genes and reconstruction of catabolic pathways by genomic and transcriptomic characteristics
Biodegradation of phenol by Candida tropicalis sp.: Kinetics, identification of putative genes and reconstruction of catabolic pathways by genomic and transcriptomic characteristics
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热带假丝酵母对苯酚的生物降解：动力学、推定基因的鉴定以及通过基因组和转录组特征重建分解代谢途径
DOI：
10.1016/j.chemosphere.2022.136443
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发表时间：
2022-09-26
期刊：
影响因子：
8.8
通讯作者：
Jia, Xiaoshan
中科院分区：
文献类型：
--
作者：
He, Yuzhe;Wang, Zhangna;Jia, Xiaoshan
Tropicalis sp。即使在高浓度或酸环境中，也以主要的生物降解能力分离。在以下con中评估苯酚的生物降解10-1750 mg l-1，菌株表现出良好的生物降解效率。最大特异性生长速率为0.660 H-1，特异性生物降解速率为0.47 mg（苯酚）[（Mg（vss）H] -1。筛选了差异表达的基因，结果揭示了能量和碳代谢的完整过程。明显的上调，包括PHEA，CATA，OXCT和FADA通过特定活性的分析来验证关键的酶。
Candida tropicalis sp. was isolated with predominant biodegradation capability to phenol compounds, even with high concentration or in acid environment. The biodegradation of phenol was evaluated at the following con-centrations 10-1750 mg L-1, the strain exhibited well biodegradation efficiency. The maximum specific growth rate was 0.660 h-1 and the specific biodegradation rates was 0.47 mg (phenol) [(mg (VSS) h]- 1. Differentially expressed genes were screened out, and results revealed a complete process of energy and carbon metabolism. The genes' arrangements and phylogenetic information showed the unique genetic characteristics of the strain. Catabolic pathways were reconstructed and some key phenol-degrading genes were obviously upregulated, including pheA, catA, OXCT and fadA. A notable detail that CMBL encoding carboxymethylenebutenolidase was speculated to be involved in a shortened pathway of phenol biodegradation, thereby contributing to the reconstruction of the novel phenol catabolic pathway through the hydrolases of dienelactone. Finally, key en-zymes were verified by the analysis of specific activity.