Biological effects of cellular stretch on human dermal fibroblasts
Biological effects of cellular stretch on human dermal fibroblasts
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细胞拉伸对人真皮成纤维细胞的生物学效应
DOI：
10.1016/j.bjps.2013.08.002
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发表时间：
2013-12-01
影响因子：
2.7
通讯作者：
Ogawa, Rei
中科院分区：
文献类型：
--
作者：
Huang, Chenyu;Miyazaki, Kunio;Ogawa, Rei
病理性瘢痕是纤维增生性皮肤病，其特征在于成纤维细胞和胶原蛋白的积累。越来越多的人认识到，它们的发展和进展可能与局部皮肤力学有关，例如拉伸。本研究评估了细胞拉伸对正常人皮肤成纤维细胞的形态和功能的影响，并探讨了可能涉及的力学转导机制。当成纤维细胞受到24小时的周期性轴向拉伸（10个周期/min（-1））时，它们比未拉伸的细胞迁移得更快，距离更长。增加的迁移导致细胞重新定向成垂直于拉伸方向。这与细胞凋亡减少和增殖不变有关。拉伸并没有增加胶原蛋白的合成，但增加了胶原蛋白的降解。这些生物学效应似乎是由整合素和Wnt机械转导途径介导的，其通过细胞-基质相互作用、细胞-细胞连接和间接细胞-细胞通信来传递机械刺激。更好地了解这种成纤维细胞的机械反应在体外将有助于开发新的干预措施，可以预防，减少甚至逆转病理性瘢痕形成和/或进展在体内。(C)2013年英国整形、重建和美容外科医生协会。由爱思唯尔有限公司出版。保留所有权利。
Pathological scars are fibroproliferative skin disorders that are characterised by the accumulation of fibroblasts and collagens. It is increasingly understood that their development and progression may be related to local skin mechanics, such as stretching. The present study evaluated the morphological and functional effects of cellular stretch on normal human dermal fibroblasts and explored the mechanotransduction mechanisms that may be involved. When fibroblasts were subjected to 24 h of cyclic axial stretching (10 cycles min(-1)), they migrated faster and for a longer distance than unstretched cells. The increased migration resulted in the cells reorienting themselves perpendicular to the direction of stretching. This was associated with reduced cellular apoptosis and unchanged proliferation. Stretching did not increase collagen synthesis but did elevate collagen degradation. These biological effects appeared to be mediated by the integrin and Wnt mechanotransduction pathways, which transmitted the mechanical stimulus via cell-substrate interactions, cell-cell junctions and indirect cell-cell communications. A better understanding of such fibroblast mechanoresponses in vitro will help the development of novel interventions that can prevent, reduce or even reverse pathological scar formation and/or progression in vivo. (C) 2013 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.