Arabidopsis thaliana leaves with altered chloroplast numbers and chloroplast movement exhibit impaired adjustments to both low and high light.
Arabidopsis thaliana leaves with altered chloroplast numbers and chloroplast movement exhibit impaired adjustments to both low and high light.
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DOI：
10.1093/jxb/ern099
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发表时间：
2008
影响因子：
6.9
通讯作者：
Harris GC
中科院分区：
文献类型：
--
作者：
Königer M;Delamaide JA;Marlow ED;Harris GC
以拟南芥（Arabidopsis thaliana）为材料，研究了叶绿体数目和大小对蓝光依赖的叶绿体运动能力、弱光下增加光吸收能力以及对光抑制敏感性的影响。叶的野生型和叶绿体数突变体的平均叶绿体数范围从120至2个叶肉细胞进行了分析。叶绿体运动的监测光透过叶片的变化。光透射率被用作叶片优化光吸收能力的指标。通过使用叶绿素a荧光测量PSII的光化学效率来确定叶片在10 °C下处理3小时强光胁迫的能力以及它们在低光下恢复的能力。叶片中的叶绿体运动是相当的，每个叶肉细胞的叶绿体数量从120到30个不等：暴露于0.1（积累反应）和100 μmol光子m−2 s−1（回避反应）后的最终光透射水平是无法区分的，叶绿体对光照强度的小幅增加反应迅速，运动动力学相似。然而，当每个叶肉细胞的叶绿体数减少到18个或以下时，积累响应显著降低。回避反应只受损的突变体与9个或更少的叶绿体，无论是在最终的传输水平和运动速度。只有突变体缺乏蓝光受体（phot 1/phot 2）或那些叶绿体数量急剧减少，严重影响回避反应表现出降低的能力，从高光胁迫恢复。
The effects of chloroplast number and size on the capacity for blue light-dependent chloroplast movement, the ability to increase light absorption under low light, and the susceptibility to photoinhibition were investigated in Arabidopsis thaliana. Leaves of wild-type and chloroplast number mutants with mean chloroplast numbers ranging from 120 to two per mesophyll cell were analysed. Chloroplast movement was monitored as changes in light transmission through the leaves. Light transmission was used as an indicator of the ability of leaves to optimize light absorption. The ability of leaves to deal with 3 h of high light stress at 10 °C and their capacity to recover in low light was determined by measuring photochemical efficiencies of PSII using chlorophyll a fluorescence. Chloroplast movement was comparable in leaves ranging in chloroplast numbers from 120 to 30 per mesophyll cell: the final light transmission levels after exposure to 0.1 (accumulation response) and 100 μmol photons m−2 s−1 (avoidance response) were indistinguishable, the chloroplasts responded quickly to small increases in light intensity and the kinetics of movement were similar. However, when chloroplast numbers per mesophyll cell decreased to 18 or below, the accumulation response was significantly reduced. The avoidance response was only impaired in mutants with nine or fewer chloroplasts, both in terms of final transmission levels and the speed of movement. Only mutants lacking both blue light receptors (phot1/phot2) or those with drastically reduced chloroplast numbers and severely impacted avoidance responses showed a reduced ability to recover from high light stress.
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影响因子：
7.4
作者：
Jeong, WJ;Park, YI;Liu, JR
通讯作者：
Liu, JR
影响因子：
2.9
作者：
Kumatani, T.;Sakurai-Ozato, N.;Takagi, S.
通讯作者：
Takagi, S.
DOI：
10.1073/pnas.0530206100
发表时间：
2003-04-01
影响因子：
11.1
作者：
Gao, HB;Kadirjan-Kalbach, D;Osteryoung, KW
通讯作者：
Osteryoung, KW
影响因子：
4.3
作者：
Königer, M;Harris, GC;Pearcy, RW
通讯作者：
Pearcy, RW
影响因子：
7.4
作者：
Luesse, Darron R.;DeBlasio, Stacy L.;Hangarter, Roger P.
通讯作者：
Hangarter, Roger P.