Treadmill exercise promotes interleukin 15 expression in skeletal muscle and interleukin 15 receptor alpha expression in adipose tissue of high-fat diet rats
Treadmill exercise promotes interleukin 15 expression in skeletal muscle and interleukin 15 receptor alpha expression in adipose tissue of high-fat diet rats
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跑步机运动促进高脂饮食大鼠骨骼肌中白细胞介素15表达和脂肪组织中白细胞介素15受体α表达
DOI：
10.1007/s12020-012-9809-6
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发表时间：
2013-06-01
期刊：
影响因子：
3.7
通讯作者：
Zhang, Jing
中科院分区：
文献类型：
--
作者：
Yang, Hongtao;Chang, Jinrui;Zhang, Jing
白细胞介素15（IL-15）最近被认为是一种参与调节脂质代谢的肌因子。研究运动训练对肥胖大鼠骨骼肌IL-15含量和脂肪组织IL-15受体（IL-15 R）表达的影响。在高脂饮食12周后，肥胖大鼠以26 m/min的速度进行跑步机跑步（每次60 min，每周5天，持续8周）。高脂饮食诱发肥胖，伴随体重、体脂和血脂谱增加。肥胖大鼠血浆和腓肠肌中IL-15免疫反应性（IL-15-ir）水平低于对照组，腓肠肌中IL-15 mRNA水平显著降低。肥胖大鼠脂肪组织中IL-15 R的mRNA和蛋白水平明显降低。与安静组相比，跑台运动组大鼠体重减轻，肌肉IL-15 mRNA表达增加，血浆和肌肉IL-15-ir水平升高。跑台运动肥胖大鼠脂肪组织中IL-15 R的mRNA和蛋白水平升高。结果表明，运动训练可以改善肥胖，逆转高脂饮食引起的肌肉IL-15和脂肪组织IL-15 R的下调。
Interleukin 15 (IL-15) has recently been proposed as a myokine involved in regulating lipid metabolism. We investigated the effect of exercise training on IL-15 content in skeletal muscle and expression of IL-15 receptor (IL-15R) in adipose tissue of obese rats. After 12 weeks of a high-fat diet, obese rats underwent treadmill running at 26 m/min (60 min each, 5 days/week for 8 weeks). High-fat diet induced obesity, with increased body weight, body fat, and lipid profile. The level of IL-15 immunoreactivity (IL-15-ir) in plasma and gastrocnemius muscle was lower in obese than control rats, and the mRNA level of IL-15 in gastrocnemius muscle was markedly decreased. The mRNA and protein levels of IL-15R in adipose tissue were markedly lower in obese rats. Compared with sedentary obese rats, treadmill running showed decreased body weight and elevated mRNA expression of IL-15 in muscle and elevated IL-15-ir level in plasma and muscle. The mRNA and protein level of IL-15R were increased in adipose tissue in treadmill running obese rats. Our results showed that exercise training improve obesity and reversed the downregulation of the IL-15 in muscle and IL-15R in adipose tissue induced by high-fat diet.