Frequent detection of the human herpesvirus 6-specific genomes in the livers of children with various liver diseases
Frequent detection of the human herpesvirus 6-specific genomes in the livers of children with various liver diseases
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DOI：
10.1128/jcm.39.6.2173-2177.2001
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发表时间：
2001-06-01
影响因子：
9.4
通讯作者：
Okada, S
中科院分区：
文献类型：
--
作者：
Ozaki, Y;Tajiri, H;Okada, S
本研究旨在调查各种肝病儿童肝脏人类疱疹病毒6型（HHV-6）感染的频率，并评估HHV-6感染在长期非乙型非丙型肝炎儿科患者中的作用。应用聚合酶链反应（PCR）和原位杂交技术检测了48例血清学证实为HHV-6感染的患者肝组织、外周血单个核细胞（PBMC）和血浆中的HHV-6基因组。48例患者中36例（75%）肝脏中可检测到HHV-6 B基因组序列，该基因组的存在与血清转氨酶活性无关。在31例受试患者中的22例（71%）的PBMC中可检测到基因组。31例患者中20例在肝脏和PBMC中均检测到HHV-6，2例在PBMC中检测到HHV-6而在肝脏中未检测到HHV-6，3例在肝脏中检测到HHV-6而在PBMC中未检测到HHV-6，6例在肝脏中均未检测到HHV-6。9例非乙型非丙型肝炎患者中有2例可检测到抗HHV-6免疫球蛋白M抗体，而22例病因明确的肝病患者中无一例检测阳性。两组患者中均未检测到无细胞病毒DNA。我们的研究结果表明，HHV-6 B是经常出现在各种肝脏疾病的儿童的肝脏，但不支持的假设，HHV-6 B的肝脏感染与长期的非B非C肝炎。
This study was performed to investigate the frequency of human herpesvirus 6 (HHV-6) infection of the liver in children with a variety of liver diseases and to evaluate the role of HHV-6 infection in pediatric patients with prolonged non-B non-C hepatitis. Detection of the HHV-6 genomes in liver, in peripheral blood mononuclear cells (PBMC), and in plasma was performed by PCR or by in situ hybridization, Liver biopsy materials from 48 patients, in whom HHV-6 infection was serologically confirmed, were available for PCR analysis. Sequences of the HHV-6B genome were detectable in the livers of 36 of 48 patients (75%), The presence of the genome was not associated with serum transaminase activities. The genome was detectable in PBMC of 22 of 31 (71%) patients tested. In these 31 patients HHV-6 was detected in both the livers and PBMC of 20, was detected in PBMC but not in the livers of 2, was detected in the livers but not in PBMC of 3, and was detected in neither of samples of 6, In situ hybridization of the livers of six patients showed the presence of the HHV-6B genome in the nuclei of hepatocytes. The anti-HHV-6 immunoglobulin M antibody was detectable in 2 of 9 of the non-B non-C hepatitis patients, whereas none of the 22 patients with etiology-defined liver diseases tested positive. Cell-free viral DNA was not detectable in either group of patients. Our results showed that HHV-6B is frequently present in the livers of children with a variety of liver diseases but do not support the assumption that HHV-6B infection of the liver is associated with prolonged non-B non-C hepatitis.