Review of current methodological approaches for characterizing microRNAs in plants.
Review of current methodological approaches for characterizing microRNAs in plants.
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DOI：
10.1155/2009/262463
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Budak H
中科院分区：
文献类型：
--
作者：
Unver T;Namuth-Covert DM;Budak H
Advances in molecular biology have led to some surprising discoveries. One of these includes the complexities of RNA and its role in gene expression. One particular class of RNA called microRNA (miRNA) is the focus of this paper. We will first briefly look at some of the characteristics and biogenesis of miRNA in plant systems. The remainder of the paper will go into details of three different approaches used to identify and study miRNA. These include two reverse genetics approaches: computation (bioinformatics) and experimental, and one rare forward genetics approach. We also will summarize how to measure and quantify miRNAs, and how to detect their possible targets in plants. Strengths and weaknesses of each methodological approach are discussed.
