Effect of a medicinal extract from Agaricus blazei Murill on gene expression in a human monocyte cell line as examined by microarrays and immuno assays
Effect of a medicinal extract from Agaricus blazei Murill on gene expression in a human monocyte cell line as examined by microarrays and immuno assays
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DOI：
10.1016/j.intimp.2005.07.007
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发表时间：
2006-02-01
影响因子：
5.6
通讯作者：
Grinde, B
中科院分区：
文献类型：
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作者：
Ellertsen, LK;Hetland, G;Grinde, B
从食用蘑菇巴西蘑菇（Agaricus blazei Muril，AbM）中提取的提取物被广泛用作对抗癌症、感染和免疫相关疾病的非处方药。推测的效果是激活免疫系统的某些部分。为了研究该效果，我们检测了提取物对人单核细胞系（THP-1）引起的基因表达的变化。使用35 k微阵列测量mRNA转录物水平的变化，并通过免疫测定来测量选择的细胞因子基因产物的变化。包括脂多糖（LPS）用于比较。AbM和LIPS都对基因表达有显著影响。与免疫功能相关的基因被选择性上调，特别是促炎基因，如白细胞介素IL 1B和IL 8。虽然AbM诱导的大多数基因也被LPS诱导，但AbM产生了独特的谱，例如，关于细胞因子IL 1A、CXCL 1、CXCL 2和CXCL 3以及PTGS 2（环加氧酶2）的mRNA的特别增加。(c)2005 Elsevier B. V.保留所有权利。
Extracts from the edible mushroom Agaricus blazei Murill (AbM) are used extensively as a non-prescription remedy against cancer, infections, and immune related diseases. The presumed effect is to activate certain parts of the immune system. In order to investigate the effect, we examined the changes of gene expression caused by the extract on a human monocyte cell line (THP-1). Changes in the levels of mRNA transcripts were measured using 35 k microarrays, and the changes in select cytokine gene products by immuno assays. Lipopolysaccharide (LPS) was included for comparison. Both AbM and LIPS had drastic effects on gene expression. Genes related to immune function were selectively up-regulated, particularly proinflammatoric genes such as the interleukins IL1B and IL8. Although most genes induced by AbM were also induced by LPS, AbM produced a unique profile, e.g., as to a particular increase in mRNA for the cytokines IL1A, CXCL1, CXCL2 and CXCL3, as well as PTGS2 (cyclooxygenase2). (c) 2005 Elsevier B.V. All rights reserved.