Interaction between P-TEFb and the C-terminal domain of RNA polymerase II activates transcriptional elongation from sites upstream or downstream of target genes
Interaction between P-TEFb and the C-terminal domain of RNA polymerase II activates transcriptional elongation from sites upstream or downstream of target genes
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DOI：
10.1128/mcb.22.1.321-331.2002
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发表时间：
2002-01-01
影响因子：
5.3
通讯作者：
Peterlin, BM
中科院分区：
文献类型：
--
作者：
Taube, R;Lin, X;Peterlin, BM
RNA聚合酶II（RNAPII）的转录延伸受正转录延伸因子B（P-TEF B）调节。P-TEFb由Cdk 9和C型细胞周期蛋白T1（CycT 1）、CycT 2a、CycT 2b或CycK组成。CycT 1和CycT 2的C-末端区域的作用仍然未知。在这份报告中，我们证明了这些序列对于P-TEFb通过DNA激活转录是必不可少的，即，当CycT 1连接在启动子和编码序列的上游或下游时。一个富含组氨酸的延伸，这是保守的CycT 1和CycT 2在这个地区，绑定的C-末端结构域的RNAPII。这种结合是随后从靶基因表达全长转录物所必需的。因此，P-TEFb可以介导增强子对转录延长的影响。
Transcriptional elongation by RNA polymerase II (RNAPII) is regulated by the positive transcription elongation factor b (P-TEFb). P-TEFb is composed of Cdk9 and C-type cyclin T1 (CycT1), CycT2a, CycT2b, or CycK. The role of the C-terminal region of CycT1 and CycT2 remains unknown. In this report, we demonstrate that these sequences are essential for the activation of transcription by P-TEFb via DNA, i.e., when CycT1 is tethered upstream or downstream of promoters and coding sequences. A histidine-rich stretch, which is conserved between CycT1 and CycT2 in this region, bound the C-terminal domain of RNAPII. This binding was required for the subsequent expression of full-length transcripts from target genes. Thus, P-TEFb could mediate effects of enhancers on the elongation of transcription.