Clonal growth of primary cultures of rabbit ear chondrocytes in a lipid-supplemented defined medium.
Clonal growth of primary cultures of rabbit ear chondrocytes in a lipid-supplemented defined medium.
复制标题
兔耳软骨细胞原代培养物在补充脂质的确定成分培养基中的克隆生长。
DOI：
10.1016/0014-4827(83)90020-4
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发表时间：
1983
影响因子：
3.7
通讯作者：
R. Ham
中科院分区：
文献类型：
--
作者：
S. Jennings;R. Ham
兔耳软骨细胞原代培养物在无血清或其他未定义添加剂的培养基中克隆生长已经实现。克隆接种物是由兔耳软骨的胶原酶消化制备的完全分化的软骨细胞悬浮液，在培养中不需要预先适应或选择。当将分离的软骨细胞接种到MCDB 104培养基中，并添加100 ng/ml成纤维细胞生长因子（FGF）、1 μg/ml胰岛素和5 μg/ml先前为人成纤维细胞开发的脂质补充剂时，分离的软骨细胞进行克隆增殖，形成大的上皮样细胞菌落。菌落在确定的培养基中生长14天，在其中心积聚可折射的软骨样基质，该基质被酸化的阿利新绿染色，尽管其数量明显少于未定义的添加剂。该系统为在确定的背景介质中对调节软骨样分化特性表型表达的因素进行详细研究开辟了道路。
Clonal growth of primary cultures of rabbit ear chondrocytes in a defined medium without serum or other undefined additives has been achieved. The clonal inoculum is a suspension of fully differentiated chondrocytes prepared by collagenase digestion of rabbit ear cartilage and used with no prior adaptation or selection in culture. When inoculated into medium MCDB 104 supplemented with 100 ng/ml fibroblast growth factor (FGF), 1 μg/ml insulin, and 5 μg/ml of a lipid supplement previously developed for human fibroblasts, the isolated chondrocytes undergo clonal multiplication to form large colonies of epithelial-like cells. Colonies grown in the defined medium for 14 days accumulate at their centers refractile cartilage-like matrix that is stained by acidified Alcian green, although the amount is significantly less than with undefined additives. This system opens the way for detailed studies, in a defined background medium, of factors that regulate phenotypic expression of cartilage-like differentiated properties.