PROTEIN-F, A FIBRONECTIN-BINDING PROTEIN, IS AN ADHESIN OF THE GROUP-A STREPTOCOCCUS STREPTOCOCCUS-PYOGENES
PROTEIN-F, A FIBRONECTIN-BINDING PROTEIN, IS AN ADHESIN OF THE GROUP-A STREPTOCOCCUS STREPTOCOCCUS-PYOGENES
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DOI：
10.1073/pnas.89.13.6172
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发表时间：
1992-07-01
影响因子：
11.1
通讯作者：
CAPARON, M
中科院分区：
文献类型：
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作者：
HANSKI, E;CAPARON, M
已表明与纤连蛋白的结合在A组链球菌化脓性链球菌与宿主上皮细胞的粘附中起重要作用;然而，链球菌纤连蛋白受体的身份一直难以捉摸。在这里，我们证明了S。化脓性链球菌感染是由蛋白F介导的，蛋白F是一种以高亲和力结合纤连蛋白的细菌表面蛋白。编码蛋白F（prtF）的基因在大肠杆菌中产生功能性纤连蛋白结合蛋白。插入突变的克隆基因产生的突变，导致纤连蛋白结合活性的损失。将该突变导入S.通过与野生型等位基因的同源重组，使化脓性链球菌染色体上的突变株不再产生蛋白F，并失去了结合纤连蛋白的能力。该突变可以通过在质粒上引入prtF来补充。缺乏蛋白F的突变体粘附呼吸道上皮细胞的能力要低得多。这些结果表明，蛋白F是一种重要的粘附素。化脓
Binding to fibronectin has been suggested to play an important role in adherence of the group A streptococcus Streptococcus pyogenes to host epithelial cells; however, the identity of the streptococcal fibronectin, receptor has been elusive. Here we demonstrate that the fibronectin-binding property of S. pyogenes is mediated by protein F, a bacterial surface protein that binds fibronectin at high affinity. The gene encoding protein F (prtF) produced a functional fibronectin-binding protein in Escherichia coli. Insertional mutagenesis of the cloned gene generated a mutation that resulted in the loss of fibronectin-binding activity. When this mutation was introduced into the S. pyogenes chromosome by homologous recombination with the wild-type allele, the resulting strains no longer produced protein F and lost their ability to bind fibronectin. The mutation could be complemented by prtF introduced on a plasmid. Mutants lacking protein F had a much lower capacity to adhere to respiratory epithelial cells. These results demonstrate that protein F is an important adhesin of S. pyogenes.