Induction of immune cell infiltration into murine SCCVII tumour by photofrin-based photodynamic therapy.
Induction of immune cell infiltration into murine SCCVII tumour by photofrin-based photodynamic therapy.
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DOI：
10.1038/bjc.1995.108
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发表时间：
1995-03
影响因子：
8.8
通讯作者：
Dougherty, G J
中科院分区：
文献类型：
--
作者：
Krosl, G;Korbelik, M;Dougherty, G J
在给予Photofrin的C3 H/HeN小鼠中生长的鳞状细胞癌SCCVII中的细胞群在光动力学光处理期间的不同时间间隔和长达8小时后进行了检查。使用间接免疫过氧化物酶和Wright染色的组合或通过流式细胞术，通过针对细胞类型特异性膜标志物的单克隆抗体鉴定切除肿瘤内存在的细胞群。基于Photofrin的光动力疗法（PDT）诱导了所治疗的肿瘤中所含的不同细胞群体的水平的显著变化。最明显的是中性粒细胞的含量迅速增加，在光处理开始后5分钟内增加了200倍。紧接着是肥大细胞水平的增加，而另一种类型的骨髓细胞，最有可能是单核细胞，在PDT后0至2小时之间侵入肿瘤。由从SCCVII肿瘤收获的巨噬细胞介导的体外培养的SCCVII肿瘤细胞的细胞溶解的检查揭示了PDT后2小时分离的肿瘤相关巨噬细胞的杀肿瘤活性的显著增加。因此，PDT诱导的骨髓细胞急性炎症性浸润似乎与免疫细胞的功能激活有关。
Cellular populations in the squamous cell carcinoma SCCVII, growing in C3H/HeN mice given Photofrin, were examined at various time intervals during the photodynamic light treatment and up to 8 h later. Cell populations present within excised tumours were identified by monoclonal antibodies directed against cell type-specific membrane markers using a combination of the indirect immunoperoxidase and Wright staining or by flow cytometry. Photofrin-based photodynamic therapy (PDT) induced dramatic changes in the level of different cellular populations contained in the treated tumour. The most pronounced was a rapid increase in the content of neutrophils, which increased 200-fold within 5 min after the initiation of light treatment. This was followed immediately by an increase in the levels of mast cells, while another type of myeloid cells, most likely monocytes, invaded the tumour between 0 and 2 h after PDT. The examination of cytolysis of in vitro cultured SCCVII tumour cells mediated by macrophages harvested from the SCCVII tumour revealed a pronounced increase in the tumoricidal activity of tumour-associated macrophages isolated at 2 h post PDT. It seems, therefore, that the PDT-induced acute inflammatory infiltration of myeloid cells into the treated tumour is associated with functional activation of immune cells.