Molecular cloning of a gene belonging to the carcinoembryonic antigen gene family and discussion of a domain model.
Molecular cloning of a gene belonging to the carcinoembryonic antigen gene family and discussion of a domain model.
复制标题
属于癌胚抗原基因家族的基因的分子克隆和域模型的讨论。
DOI：
10.1073/pnas.84.9.2965
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发表时间：
1987
影响因子：
11.1
通讯作者：
Shively,JE
中科院分区：
文献类型：
--
作者：
Thompson,JA;Pande,H;Paxton,RJ;Shively,L;Padma,A;Simmer,RL;Todd,CW;Riggs,AD;Shively,JE
癌胚抗原（CEA）是一种重要的糖蛋白，是结肠癌的肿瘤标志物。免疫学和生物化学研究表明，它与许多其他糖蛋白密切相关，共同构成一个基因家族。基于我们的CEA和NCA（非特异性交叉反应抗原）蛋白序列数据和人类密码子使用，我们使用长寡核苷酸探针（42-54个核苷酸）克隆了该基因家族的一个成员。得到的克隆（lambda 39.2）与6个探针杂交，插入15千碱基。该基因的5'端包含在一个2700碱基对的EcoRI片段中，该片段与6个合成探针中的5个杂交。5'端区测序揭示了一个外显子和两个假定的内含子边界的位置和结构。外显子编码NCA的部分前导序列和nh2末端107个氨基酸。使用含有编码序列的两个lambda 39.2片段作为探针，对人正常和肿瘤DNA进行Southern blot分析，发现CEA家族存在9-11个基因。这里描述的一个限制性片段已被Zimmermann等人使用[Zimmermann， W., Ortlieb， B., Friedrich， R. & von Kleist， S. (1987) Proc. Natl.]。学会科学。[USA 84, 2960-2964]分离CEA部分cDNA克隆。利用我们的蛋白质序列数据验证了该克隆的身份[Paxton， R., Mooser， G., Pande， H., Lee， T.D. & Shively， J.E. (1987) Proc. Natl.]。学会科学。《美国科学》第84卷，920-924页。基于CEA序列数据和NCA外显子序列数据，讨论了CEA的结构域结构。该基因家族可能与神经细胞粘附分子和α 1 b -糖蛋白有共同的祖先，可能是免疫球蛋白超家族的一个家族。
Carcinoembryonic antigen (CEA) is a glycoprotein important as a tumor marker for colonic cancer. Immunological and biochemical studies have shown it to be closely related to a number of other glycoproteins, which together make up a gene family. We have cloned a member of this gene family by using long oligonucleotide probes (42-54 nucleotides) based on our protein sequence data for CEA and NCA (nonspecific cross-reacting antigen) and on human codon usage. The clone obtained (lambda 39.2) hybridizes with six probes and has a 15-kilobase insert. The 5' end of the gene is contained within a 2700-base-pair EcoRI fragment, which hybridizes with five of the six synthetic probes. Sequencing of the 5' end region revealed the location and structure of one exon and two putative intron boundaries. The exon encodes part of the leader sequence and the NH2-terminal 107 amino acids of NCA. Southern blot analysis of human normal and tumor DNA, using as probes two lambda 39.2 fragments that contain coding sequences, suggests the existence of 9-11 genes for the CEA family. One of the restriction fragments described here has been used by Zimmermann et al. [Zimmermann, W., Ortlieb, B., Friedrich, R. & von Kleist, S. (1987) Proc. Natl. Acad. Sci. USA 84, 2960-2964] to isolate partial cDNA clones for CEA. The identity of this clone was verified with our protein sequence data [Paxton, R., Mooser, G., Pande, H., Lee, T.D. & Shively, J.E. (1987) Proc. Natl. Acad. Sci. USA 84, 920-924]. We discuss a domain structure for CEA based on the CEA sequence data and the NCA exon sequence data. It is likely that this gene family evolved from a common ancestor shared with neural cell adhesion molecule and alpha 1 B-glycoprotein and is perhaps a family within the immunoglobulin superfamily.
DOI：
--
发表时间：
1972
期刊：
影响因子：
--
作者：
G. Birnie
通讯作者：
G. Birnie
影响因子：
8.8
作者：
M. Moore
通讯作者：
M. Moore