Changed gene expression in brains of mice exposed to traffic in a highway tunnel
Changed gene expression in brains of mice exposed to traffic in a highway tunnel
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DOI：
10.3109/08958378.2012.714004
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发表时间：
2012-08-01
影响因子：
2.1
通讯作者：
Panis, Luc Int
中科院分区：
文献类型：
--
作者：
Bos, Inge;De Boever, Patrick;Panis, Luc Int
内容：空气污染已被建议对brain.Objective的影响：目的是评估炎症相关基因在小鼠的大脑中的表达，已暴露5天的一个良好的特征交通污染的环境，即高速公路tunnel.Materials和方法：20只C57 BL 6小鼠被随机分配到4组，每组5只动物。两组被放置在隧道中5天（平均PM 2.5，55.1 μ g/m3，平均元素碳，EC 13.9 μ g/m3），在有或没有过滤器的笼子里，两个对照组被安置在隧道外。在末次暴露日后24小时内对动物进行评估。通过支气管肺泡灌洗（BAL）和组织学评估肺损伤和炎症。测定外周血白细胞和凝血参数。嗅球和海马进行了分析，炎症基因和脑源性神经营养因子（BDNF）的表达的变化。结果和讨论：虽然碳颗粒在暴露小鼠的肺泡巨噬细胞丰富，在非暴露小鼠缺席，没有证据表明肺部或全身炎症。在暴露小鼠的海马中，参与炎症反应的基因（COX 2，NOS 2，NOS 3和NFE 2L 2）的表达增加。在嗅球中，IL 1 α，COX 2，NFE 2L 2，IL 6，和BDNF的下调被发现。结论：虽然这种短期暴露于交通相关的污染不会引起肺部或全身炎症，炎症基因的表达在不同的脑区受到影响。嗅球中BDNF表达的减少表明对交通相关空气污染的脑神经营养支持降低。
Context: Air pollution has been suggested to have an impact on the brain.Objective: The objective was to assess the expression of inflammation-related genes in the brains of mice that had been exposed for 5 days to a well-characterized traffic-polluted environment, i.e. a highway tunnel.Materials and methods: Twenty C57BL6 mice were randomly allocated to four groups of five animals. Two groups were placed in the tunnel for 5 days (mean PM 2.5, 55.1 mu g/m(3), mean elemental carbon, EC 13.9 mu g/m(3)) in cages with or without filter, two control groups were housed outside the tunnel. Animals were assessed within 24 hours after the last exposure day. Lung injury and inflammation were assessed by bronchoalveolar lavage (BAL) and histology. Blood leukocytosis and coagulation parameters were determined in peripheral blood. The olfactory bulb and hippocampus were analyzed for changes in expression of inflammatory genes and brain-derived neurotrophic factor (BDNF).Results and discussion: Although carbon particles were abundant in alveolar macrophages of exposed mice and absent in non-exposed mice, there was no evidence of pulmonary or systemic inflammation. There was an increased expression of genes involved in inflammatory response (COX2, NOS2, NOS3, and NFE2L2) in the hippocampus of the exposed mice. In the olfactory bulb, a downregulation was found for IL1 alpha, COX2, NFE2L2, IL6, and BDNF.Conclusion: Although this short-term exposure to traffic-related pollution did not induce pulmonary or systemic inflammation, the expression of inflammatory genes was affected in different brain areas. The decreased BDNF expression in the olfactory bulb suggests lower brain neurotrophic support in response to traffic-related air pollution.